Korean J Nephrol.  2011 Sep;30(5):452-458.

Effects of Lipoxygenase Inhibitor on Diabetic Nephropathy in Rats: Decreasing Proteinuria and Preserving Renal Function

• Chung HC
• Kim IY
• Kim SR
• Son J
• Lee DW
• Song SH
• Seong EY
• Kwak IS
• Lee SB

Abstract

PURPOSE
Oxidative stress leads to an increased production of lipoxygenase derivatives in diabetic nephropathy. Thus, we hypothesized that lipoxygenase inhibitor, nordihydroguaiaretic acid (NDGA), ha the effects of decreasing proteinuria and preserving renal function in streptozotocin (STZ)-induced diabetic rats.
METHODS
45 Sprague-Dawley rats were divided into three groups; (A) treatment with lipoxygenase inhibitor, NDGA in diabetic nephropathy rats, (B) treatment with dimethyl sulfoxide (DMSO) as a vehicle in STZ-induced diabetic rats, (C) normal control group with subcutaneous injection of normal saline. Diabetes was induced by a single intraperitoneal injection of STZ (65 mg/kg) in rats of group A and B. After the 4th week of STZ injection, NDGA (10 mg/kg) and DMSO were given subcutaneously for another 4 weeks in group A and B respectively.
RESULTS
The NDGA-treated diabetic rats exhibited significantly decreased urinary albumin excretion. Serum creatinine and blood urea nitrogen concentrations were increased in both group A and B, and tend to be higher in group B than group A. Twenty-four-hour urine creatinine clearances were increased in both group A and B after injection of STZ. Pathologic alterations of kidney were observed after injection of STZ, and then attenuated after administration of NDGA.
CONCLUSION
These results suggest the potential of lipoxygenase inhibitor as a complementary therapy for the prevention and treatment of diabetic nephropathy.

Keyword

Diabetic nephropathy; Proteinuria; Nordihydroguaiaretic acid

MeSH Terms

Animals
Blood Urea Nitrogen
Creatinine
Diabetic Nephropathies
Dimethyl Sulfoxide
Injections, Intraperitoneal
Injections, Subcutaneous
Kidney
Lipoxygenase
Nordihydroguaiaretic Acid
Oxidative Stress
Proteinuria
Rats
Rats, Sprague-Dawley
Safrole
Streptozocin
Creatinine
Dimethyl Sulfoxide
Lipoxygenase
Nordihydroguaiaretic Acid
Safrole
Streptozocin