Korean J Hematol.  2007 Mar;42(1):15-23. 10.5045/kjh.2007.42.1.15.

Susceptibility of Leukemia according to the Genotype of Minor Histocompatibility Antigens in a Korean Population

• Park MJ
• Choi HB
• Yoon HY
• Choi EJ
• Kim SY
• Kim HJ
• Eom KS
• Lee S
• Kim DW
• Lee JW
• Min WS
• Kim CC
• Kim TG

• KMID: 2252264
• DOI: http://doi.org/10.5045/kjh.2007.42.1.15

Abstract

BACKGROUND: In the search for susceptibility genes responsible for leukemia, genetic studies involving HLA association have been in progress extensively since the first report on its effect on the disease. Here we investigated the genetic associations of different leukemias with 4 autosomal mHags, HA-1, -2, -8 and HB-1. In particular, HB-1 is one of the leukemia-associated minor histocompatibility antigens (mHags) that is significantly expressed by Epstein-Barr virus-transformed- and tumor cells of all B lineage acute lymphoblastic leukemia (ALL).
METHODS
A simultaneous genotyping method using PCR sequence-specific primers against HA-1, -2, -8 and HB-1 was developed, and their allelic frequencies in 139 healthy controls and 36 leukemia patients were observed. To compare genotype, phenotype, and gene frequencies of mHags with healthy controls, leukemia patients were classified into sub groups of ALL, acute myeloid leukemia (AML), and chronic myeloid leukemia (CML).
RESULTS
The genotype frequencies of HA-1, -2 and -8 were not significantly different from healthy controls in every group of leukemia patients. However, the HB-1 H genotype was significantly increased in leukemia patients (P=0.03, OR=1.82, CI=1.08~3.06), particularly in AML (P=0.01, OR=2.4, CI=1.21~4.76) as compared with healthy controls.
CONCLUSION
Our results suggested that the genotype of HB-1 H may be associated with leukemia, particularly with AML. In further study, it is necessary to confirm the association of HB-1 with other leukemias in a larger group of patients, and to identify the underlying mechanism of HB-1 responsible for the occurrence of leukemia.

Keyword

Leukemia; Minor histocompatibility antigens; Korean

MeSH Terms

Gene Frequency
Genotype*
Humans
Leukemia*
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Leukemia, Myeloid, Acute
Minor Histocompatibility Antigens*
Phenotype
Polymerase Chain Reaction
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Minor Histocompatibility Antigens

Figure

• 

  Fig 1. Genotyping of HA-1, -2, -8 and HB-1 by PCR-SSP. Lane 1: H type for HA-1, lane 2: R type for HA-1, lane 3: V type for HA-2, lane 4: M type for HA-2, lane 5: R type for HA-8, lane 6: P type for HA-8, lane 7: H type for HB-1, lane 8: Y type for HB-1. Each type of mHags HA-1, -2, -8 and HB-1 of the three samples was as follows; sample 1: HH (HA-1), VM (HA-2), PP (HA-8), HH (HB-1); sample 2: RR (HA-1), VM (HA-2), RP (HA-8), YY (HB-1); sample 3: HR (HA-1), VV (HA-2), RR, (HA-8), HY (HB-1).

• 

  Fig. 2 Allelic frequencies of HA-1, -2, -8 and HB-1 in healthy controls (n=139) and patients with leukemia (n=36) in the Korea.

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