Korean J Gynecol Oncol Colposc.  1990 Mar;1(1):8-25.

Effect of Retinol Acetate on Adenosis of Vaginal and Cervical Epithelium Induced by Early Neonatal Treatment with Diethylstilbestrol (DES) in Mice

• Ahn WS
• Han SG
• Namkung SE
• Song SK
• Lee HY
• Kim SJ

Abstract

Prevention by retinol acetate of the occurrenrce of diethylstilbestrol(DES) induced persistent vaginal and uterine changes was studied in neonatal ICR strain mice. The mice were devided into six groups. DES injected group received five daily injectiona of 20ug DES alone from the day of birth. In the DES plus retinol acetate-injected groups, daily injections of 20ug DES together with 100 I,U. or 200 I.U. retinol acetate were given tu mice for 5 days from the day of birth respectively. Another group of mice was first given daily injections of 20ug DES for 5 days from the day of birth and then given five daily injections af 200 I.U. retinnl acetate from 15th postnatal day. In addition, daily injectiuns of 200 I.U. retinol aeetate only and of 0.02ml sesame oil only were given to mice for 5 days from the day of birth respectively. The mice were sacrifieecl at 5 (lays, 30 days, and 120 days separately. Histological observation was made on vaginae ancI uteri. Cells at metaphae per 500 basal cells were calculated in two sectinns of each of the cranial and candal portions of the vaginal epithelium and in section of uterine epithelium. Number of cell layers of the vaginal and uterine epithelium was counted. The occorrence cif the atypical epithelial change such as adenosis, adensis-like lesion and keratinizatioa was also examined. The results were as follows: 1) In the 120-day-old mice receiving neonatal DES injection, the occurrence of adenosis significantly inereased(P<0.001) compared with neonatally DES-injected, 5-day-old or 30-day-old mice. 2)In the DES plus retinol acetate-injected groups, the occurrence of adenosis significantly decreased(P<0.01), and 1000I.U.(2001.U./day) retinol acetate-injected group had more blocking trend on the occurrence of adenosis compared with 500I.U.(100I.U./day) retinol acetate-injected groups, the mitotic rate significantly increased(P<0.05) compared with 30-day-old mice of DES-injected group,(P<0.05) compared with DES-injected mice aged 120 days. The mitotic rate was more significantly decreased in the injection of DES together with 1000I.U.(2001.U./day) retinol acetate(P<0.001) than that of DES together 500I.U.(100I.U./day) retinol acetate among mice aged 120 days. 4) There was no difference of the number of cell layers in both cranial and caudal portions of vaginal epithelium between DES plus retinol acetate injected mice aged 30 days and DES-injected mice aged 30 days. In the 120-day-old mice, DES plus retinol acetate-injected group showed a significant decrease of the number of cell layers in both cranial and caudal portions of vaginal epithelium(P<0.001) compared with DES-injected group. 5) Two groups which had received injections either of 1000I.U.(2001.U./day) retinol acetate from the day of birth or of 1000I.U.(2001.U./day) retinol acetate from the 15th postnatal day showed no significant difference in the blocking effect of the occurrence of DES induced vaginal adenosis. However, of the two groups, the mitotic rate and the number of cell layers in vaginal epothelium are more significantly decreased in the former than the latter(P<0.01). The present study, therefore, suggests that rerinol acetate has a blocking effcet on occurrence of DES-induced atypical epithelial changes in vaginae and uteri, and the blocking effect depends upon dose of retinol acetate and date.

MeSH Terms

Animals
Diethylstilbestrol*
Epithelium*
Mice*
Parturition
Sesame Oil
Uterus
Vagina
Vitamin A*
Diethylstilbestrol
Sesame Oil
Vitamin A