Korean J Hepatobiliary Pancreat Surg.  2002 Jun;6(1):42-47.

Liver Fibrosis by Bile Duct Sclerosis with Ethanol: a New Experimental Model for Liver Fibrosis

Affiliations
  • 1Department of Surgery, College of Medicine, Chungbuk National University, Cheongju, Korea. jwchoi@med.chungbuk.ac.kr
  • 2Department of Pathology, College of Medicine, Chungbuk National University, Cheongju, Korea.
  • 3Department of Internal Medicine, College of Medicine, Chungbuk National University, Cheongju, Korea.

Abstract

BACKGROUND/AIMS: Common bile duct ligation will induce cholestatic fibrosis or cirrhosis in the rat. However, the degree of histologic changes following ligation of the common bile duct appears to vary widely due to recanalization of the bile duct in the distensible extrahepatic system. The purpose of this study is to compare liver damages induced by traditional simple ligation of bile duct with those induced by ethanolic sclerosis of bile duct and ligation.
METHODS
Twenty rats of 7 weeks-aged Spraugue-Dawley (body weight 200~250 gm) were divided into 2 groups, common bile duct ligation (BDL group n=10), and 60% ethanol infusion into the common bile duct before bile duct ligation (ETL group n=10). After 7 weeks, all animals were sacrificed. Gross findings, histologic findings (Ki-67 and Masson- trichrome staining), and liver function test were compared.
RESULTS
During the experiment, 5 rats of BDL group and 4 rats of ETL group died. In the operative findings, diameter of bile duct in BDL group was dilatated 8~15 mm, but there was no dilatation in ETL group. In the histologic findings of extrahepatic bile duct, epithelial cells were maintained well in BDL group, but they were all cast off or destroyed in ETL group. Liver fibrosis and cell proliferation are more prominent in the ETL group. Also, ETL group showed worse liver function test than that of BDL group.
CONCLUSION
Liver fibrosis or cirrhosis by prolonged bile duct ligation is a well-known experimental model. In this study, we demonstrated that a new method of bile duct ligation (ethanol infusion before bile duct ligation) which abolishes bile duct distention accompanied with simple bile duct ligation is more effective to produce liver fibrosis or cirrhosis.

Keyword

Bile duct ligation; Liver fibrosis; Sclerosis of bile duct

MeSH Terms

Animals
Bile Ducts*
Bile Ducts, Extrahepatic
Bile*
Cell Proliferation
Common Bile Duct
Dilatation
Epithelial Cells
Ethanol*
Fibrosis
Ligation
Liver Cirrhosis*
Liver Function Tests
Liver*
Models, Theoretical*
Rats
Sclerosis*
Ethanol
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