Korean J Gastroenterol.  2000 May;35(5):610-620.

Effect of Doses and Formulations of Bacterial Lipase on Intestinal Transit in Canine Exocrine Pancreatic Insufficiency

• Chung JP
• Chung JB
• Lee SJ
• Park HJ
• Moon BS
• Song SY
• Lee KS
• Lee SI
• Moon YM
• Kang JK
• DiMagno EP

Abstract

BACKGROUND/AIMS
We determined in canine exocrine pancreatic insufficiency (EPI) when fed a high fat meal if 1) lipolytic activity as bacterial lipase (BL) alters intestinal transit (IT) dose dependently, 2) different formulations of BL have different dose responses, 3) correction of steatorrhea is related to IT.
METHODS
Five dogs underwent ligation of a high fat meal (fat 43%) was eaten. Powder or microtablet BL were given at doses ranging from 0 to 400x103 IU. IT of liquid and solid meal markers was evaluated by total delivery (% total amounts delivered to ileum), AUC (area under curve), T10 and T4 (minutes for 10% or 4% of ingested markers to travel from duodenum to ileum).
RESULTS
IT of the solid meal marker was not related to doses or formulations of BL. However, slowing of IT of the liquid meal marker was dose, but not formulation dependent (total delivery, p=0.0004; AUC, p=0.0017; T10, p=0.0245). Greater fat absorption was associated with slower IT of the liquid but not the solid meal marker (r2=0.262, p=0.0213).
CONCLUSIONS
In dogs with EPI fed a high fat diet, slower IT of liquids is dose- but not formulation-dependent and slowing of IT of liquids correlates with greater fat absorption.

Keyword

Exocrine pancreatic insufficiency; Bacterial lipase; Intestinal transit; Fat absorption

MeSH Terms

Absorption
Animals
Area Under Curve
Diet, High-Fat
Dogs
Duodenum
Exocrine Pancreatic Insufficiency*
Ligation
Lipase*
Meals
Steatorrhea
Lipase