Gut Liver.  2015 Sep;9(5):657-664. 10.5009/gnl14188.

Hepatitis B Surface Antigen Quantification across Different Phases of Chronic Hepatitis B Virus Infection Using an Immunoradiometric Assay

Affiliations
  • 1Department of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Seoul, Korea.
  • 2Department of Internal Medicine, Seoul Metropolitan Government Seoul National University Boramae Medical Center, Seoul, Korea. drwon1@snu.ac.kr
  • 3Department of Nuclear Medicine, Seoul National University Bundang Hospital, Seongnam, Korea.

Abstract

BACKGROUND/AIMS
Quantification of hepatitis B surface antigen (HBsAg) is an emerging serologic test and may be useful for identifying treatment strategies for chronic hepatitis B (CHB). This study aimed to evaluate HBsAg titers during the natural course of CHB and identify correlations between HBsAg titers and hepatitis B virus (HBV) DNA concentrations across different CHB phases measured using an immunoradiometric assay (IRMA).
METHODS
CHB phases were defined on the basis of HBV DNA concentrations, the presence of hepatitis B e antigen/antibody (HBeAg/Ab) and serum alanine aminotransferase levels. Serum HBsAg titers and paired HBV DNA concentrations in the different phases of CHB were compared using 627 serum samples.
RESULTS
Mean HBsAg titers were significantly higher in the immunotolerant (IT) phase and immunoreactive (IR) HBeAg-positive phase than in the low-replicative (LR) and HBeAg-negative CHB (ENH) states. The correlation between HBsAg titers and HBV DNA concentrations was modest in the IT (n=36, r=0.804, p<0.001) and IR (n=48, r=0.773, p<0.001) phases, and it was poor in the LR state (n=116, r=0.289, p=0.002); however, no significant correlation was observed in the ENH state (n=67, r=0.146, p=0.237) or in the oral nucleos(t)ide analogue-treated group (n=267).
CONCLUSIONS
HBsAg quantification using IRMA might be useful for discriminating different CHB phases and different stages of chronic liver disease.

Keyword

Hepatitis B virus; Hepatitis B surface antigens; Immunoradiometric assay; DNA

MeSH Terms

Adult
Alanine Transaminase/blood
Biomarkers/blood
DNA, Viral/*blood
Disease Progression
Female
Hepatitis B Surface Antigens/*blood
Hepatitis B e Antigens/blood
Hepatitis B virus/*genetics/immunology
Hepatitis B, Chronic/*immunology
Humans
*Immunoradiometric Assay
Male
Middle Aged
Seoul
Viral Load
Virus Replication
Alanine Transaminase
Biomarkers
DNA, Viral
Hepatitis B Surface Antigens
Hepatitis B e Antigens
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