The Effect of External Beam Radiation on Neointimal Formation in the Rat Carotid Injury Model

Kim, Han Soo; Yoon, Myeong Ho; Oh, Young Taek; Chun, Mi Sun; Park, Chan Hee; Kim, Jung Sun; Tahk, Seung Jea; Choi, Byung Il; Kim, Young Mi; Park, Kyung Bae

Abstract

BACKGROUND
Restenosis after successful percutaneous transluminal coronary angioplasty remains a major obstacle to the long-term success of the procedure. Uncontrolled proliferation and extracelluar matrix synthesis in response to mechanical injury are important contributors to this proces. External beam radiation or gamma radiation affects self-renewing tissues by arresting cell division, and therefore limits proliferation by reducing the number of clonal progenitors. The purpose of this study was to determine whether external beam radiation (EBR) could reduce the extent of neointimal formation after balloon injury in the rat carotid injury model and, if it could, to define the minimum effective dose.
METHODS
32 Sprague-Dawley rats (mean weight : 370+/-78g) underwent carotid injury by using 2F Fogarty balloon and EBR with doses ranging from 5 to 20 Gy. Rats were sacrificed after 2 weeks. The arteries were perfusin-fixed in paraformaldehyde. The dose was specified to a depth of 15mm. Histomorphometry was performed to compare external elastic lamina (EEL) area (mm2), lumen area (mm2)and neointimal area (mm2 of the injured segments.
RESULTS
EEL area showed no significant difference in each different radiation group. Low dose EBR (5 Gy and 10Gy) had no significant impact on neointimal hyperplasia in rat carotid injury model. However, in high dose group (15Gy and 20Gy) neointimal area was significantly reduced (p<0.01) indicating high dose EBR markedly prevented neointimal hyperplasia.
CONCLUSION
Radiation therapy may be an effective adjunctive method for reducing the restenosis rate after successful angioplasty in the rat carotid injury model.