Evaluation of Automated Chemiluminescent Immunoanalyzer Immulite 2000

Park, Do Sim; Lee, Young Jin; Cho, Ji Hyun

Abstract

BACKGROUND: The Immulite 2000 (Diagnostic Products Corp., LA, USA) is a new fully automated chemiluminescent immunoanalyzer. We evaluated the analytical performance of the Immulite 2000.
METHODS
We assayed for five tumor markers (alpha-fetoprotein, carcinoembryonic antigen, human chorionic gonadotropin, prostate-pecific antigen, and free prostate-pecific antigen), four reproductive hormones (estradiol, follicle-timulating hormone, luteinizing hormone, and prolactin) and ferritin. Within-un and between-ay precision, linearity, recovery rates, interference, detection limits, comparison with other machines were evaluated. The carry over and hook effct for HCG (human chorionic gonadotropin) were also evaluated.
RESULTS
Within-un and between-ay precisions (CVs) were less than 7.0%, 9.1% respectively. The linearity was good for all assays. The recovery rates were also acceptable. No significant analytical interference was caused by hemoglobin (up to 2,000 mg/L), triglyceride (up to 46 mmol/L), and bilirubin (up to 257 micromol/L) for all assays except for estradiol at high concentration of bilirubin. No sample-to-ample carry over was detected. A hook effect was not detected for HCG at the level of 909,000 IU/L.
CONCLUSIONS
The Immulite 2000 was shown to have an satisfactory analytical performance for modern layout of the instrument, ease of use, minimal maintenance, as well as the analytical labor efficient productivity of wide variety of analytes. So we concluded that the system was highly suitable for implementation in an automated clinical laboratory.