The Relationship of Neutrophil Function with Total Antioxidant Status and Lipid Peroxidation in Diabetes Mellitus

Hwang, Sang Hyun; Lee, Kyung Shin; Chun, Sail; Min, Won Ki; Park, Chan Jeoung; Hong, Sung Kwan

Abstract

BACKGROUND
Functional defects of neutrophils are regarded as a major factors for the susceptibility to bacterial infection in diabetes. The attribution of low antioxidant status, which is shown in uncontrolled diabetic patients, to the dysfunction in neutrophils has not been illucidated. We purposed to evaluate the correlation of respiratory burst activity in neutrophils to total antioxidant status (TAS) and lipid peroxidation. MATERIALS: Neutrophil respiratory burst activity (NRBA) before and after stimulation by PMA was measured by flowcytometry in diabetic patients with poor glucose control (n=10, HbA1C > 8.0%) and good glucose control (n=11, HbA1C < or = 6.4%) and non-diabetic subjects (n=18). TAS and lipid peroxidation assay were measured by colorimetry.
RESULTS
The results of HbA1C, TAS and lipid peroxidation of diabetic patients with poor glucose control were 11.6+/-9.2%, 1.407+/-0.041 mmol/L, 2.66+/-1.84 M, and with good glucose control were 5.7+/-0.5%, 1.440+/-0.061 mmol/L, 2.64+/-1.29 M, respectively. In non-diabetic subjects were 5.2+/-0.4%, 1.464+/-0.003 mmol/L, 2.07+/-1.18 M, respectively. NRBA of diabetic patients with poor glucose control before and after stimulation by PMA was 0.858+/-0.001 and 0.897+/-0.007 of non-diabetic subjects, respectively. NRBA of diabetic patients with good glucose control before and after stimulation by PMA was 0.832+/-0.002, 0.949+/-0.002 of non-diabetic subjects, respectively. TAS of diabetic patients with poor glucose control was significantly lower than that of non-diabetic subjects (P<0.05). NRBA were significantly decreased both before and after stimulation by PMA in poor glucose control (P<0.05). TAS and NRBA after stimulation by PMA of patients with good glucose control was not statistically significant and NRBA only before stimulation by PMA was significantly decreased (P<0.05). Significant positive correlations were evident between NRBA and total antioxidant activity (r=0.507, P<0.05).
CONCLUSION
Only NRBA before stimulation by PMA of diabetic patients with good glucose control was significantly decreased, but NRBA of diabetic patients with poor glucose control was significantly decreased before and after stimulation by PMA and significantly correlated with TAS.