Korean J Anat.
2000 Jun;33(3):365-372.
The Effect of reconstituted basement membrane on the expression of the anterior pituitary hormones of the rat
- Affiliations
-
- 1Department of Anatomy, College of Medicine, Seoul National University, Korea.
- 2Department of Radiology, Gachon Medical School, Korea.
- 3Department of Anatomy, College of Medicine, Dankook University, Korea.
- 4Department of Pediatrics, College of Medicine, Seoul National University, Korea.
Abstract
- This study was performed to investigate the effect of Matrigel, a reconstituted basement membrane, on the expression of the anterior pituitary hormones in culture. Rat pituitary cells cultured for 6 days on Matrigel showed 3-dimensional, lobular structures with connecting cells while those on plastic showed flat, polygonal cells forming a monolayer. Western blot analysis showed that prolactin (PRL) content in the anterior pituitary cells was higher compared to those cultured on plastic. In comparison, TSH expression was not increased in cultures on Matrigel. The total cell number and the proportion of fibroblasts was decreased. These results suggested that Matrigel is a useful culture substrate for the enhanced expression of PRL but not for TSH. Further studies are needed in order to find a useful culture substrate for TSH cells.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- A Case of Acromegaly Caused by Mixed Gangliocytoma-Adenoma of the Pituitary Gland
- Hypopituitarism Presenting as Osteoporotic Fracture after Cured Tuberculous Meningitis
- Regulation of Estrogen Receptor mRNA in Rat Anterior Pituitary Gland
- Pituitary Hormones and Neuropeptides
- Anionic sites in rat alveolar basement membrane
