J Korean Ophthalmol Soc.  2011 Jun;52(6):671-678. 10.3341/jkos.2011.52.6.671.

Effects of Mitomycin C on Scleral Collagen Fibrils According to Atomic Force Microscopy

• Lee HJ
• Choi S
• Cheong Y
• Jung GB
• Jin KH
• Park HK
• Lee SJ

• KMID: 2214638
• DOI: http://doi.org/10.3341/jkos.2011.52.6.671

Abstract

PURPOSE
To investigate the effects of mitomycin C on the scleral collagen surfaces using atomic force microscopy (AFM).
METHODS
Two non-contact mode AFM machines were used to observe changes in the morphological characteristics of human scleral surfaces before and after one, three, and five minutes of 0.02% mitomycin C application. Based on AFM topography and deflection images of the collagen fibril, the morphological characteristics of scleral fibrils including the fibril diameter and D-period were measured using the line profile.
RESULTS
The sclera collagen fibril treated with 0.02% mitomycin C for one minute did not show any significant increases in mean fibril diameter (155.04 +/- 17.46 nm) or mean D-periodicity (70.02 +/- 3.33 nm), compared to those of the control group. However, the scleral collagen fibrils treated with 0.02% mitomycin C for three and five minutes showed significant increases in mean fibril diameter (182.33 +/- 16.33 nm, 199.20 +/- 12.40 nm, respectively) and mean D-periodicity (70.27 +/- 13.66 nm, 72.75 +/- 19.32 nm, respectively), compared to those of the control group.
CONCLUSIONS
The present study examined the structural changes in the scleral collagen fibrils before and after mitomycin C application according to atomic force microscopy. The results indirectly suggest that three or more minutes of 0.02% mitomycin C application affects the morphology of scleral collagen.

Keyword

Atomic force microscopy; Collagen fibrils; Diameter; D-banding; Human sclera; Mitomycin C

MeSH Terms

Collagen
Humans
Microscopy, Atomic Force
Mitomycin
Sclera
Collagen
Mitomycin

Figure

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  Figure 1. (A) Atomic force microscopy (AFM) block diagram, and (B) force vs. distance and relative zone modes.

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  Figure 2. Representative atomic force microscopy topography images of the dehydrated human sclera in various scan sizes such as (A) 20 × 20 µm2, (B) 5 × 5 µm2, and (C) 1 × 1 µm2.

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  Figure 3. Representative three-dimensional images of the dehydrated human sclera in various scan sizes such as (A) 20 × 20 µm2, (B) 5 × 5 µm2, and (C) 1 × 1 µm2.

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  Figure 4. Representative examples of the diameter (C) and D-banding (D) measurements using line profiling plots on AFM topography (A) and deflection images (B) of the dehydrated scleral collagen fibrils in a scan size of 5 × 5 μ m2.

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  Figure 5. Representative AFM topography (A), deflection (B), and three-dimensional images (C) of the dehydrated scleral collagen fibrils in a scan size of 5 × 5 μ m2.

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  Figure 6. Representative AFM topography (A), deflection (B), and three-dimensional images (C) of the dehydrated scleral collagen fibrils with 0.02% mitomycin C application for 5 minutes in a scan size of 5 × 5 μ m2.

Reference

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