J Korean Surg Soc.  2000 Mar;58(3):299-306.

Donor Cell Repopulation after Bone Marrow Transplantation and Heterotopic Partial Liver Transplantation in Rats Prework for Chimerism

• Yoon MH
• Kim MH
• Hwang HY
• Chang MW
• Woo SH
• Shin DH
• Lee CH
• Park YH

Abstract

PURPOSE: Improvements in the prevention or control of rejection of kidneys and livers have been largely interchangeable and then applicable. However, the mechanism by which antirejection treatment permits any of these grafts to be accepted has been an immunological enigma. Recently, the exchange of migratory leukocytes between the transplant and the recipient, with consequent long-term cellular chimerism in both has been the basis for acceptance of all whole-organ allografts and xenografts.
METHODS
The donors of liver transplants were male Lewis rats weighing 100-150 g in all experiments groups. Male Brown Norway rats were the experimental group and female Lewis were the control group. Heterotopic partial liver transplantation was performed by Lee's method without arterial reconstruction. All procedures were performed under ether anesthesia. Bone marrow was taken from the tibias and femurs and was processed in RPMI 1640. The cell counts of suspensions were 2.5x10(8) per experiment. Genomic DNA prepared from peripheral blood and various tissues. Male Lewis Sry-specific oligonulceotide primers were used. RESULTS: In allogenic liver transplantation with bone marrow transplants (LEW-BN), donor cells were detected in the liver, and the spleen by day 7. However, in rejection cases, donor cell were not detected in any tissues. In isografted transplants (LEW-LEW), after bone marrow transplantation, donor cells were found in lymph nodes, the liver, and peripheral blood. In isografted transplants (LEW-LEW), after liver transplantation donor, cells were only found in the grafts. CONCLUSION: In allogenic liver transplantation with bone marrow transplantation, chimerism induction was augmentedwith bone marrow-derived stem cells. Therefore, it is necessary to have many samples to investigate more precisely chimerism and rejection after liver transplantation with bone marrow transplantation.

Keyword

Chimerism; Liver transplantation; Bone marrow transplantation

MeSH Terms

Allografts
Anesthesia
Animals
Bone Marrow Transplantation*
Bone Marrow*
Cell Count
Chimerism*
DNA
Ether
Female
Femur
Heterografts
Humans
Isografts
Kidney
Leukocytes
Liver Transplantation*
Liver*
Lymph Nodes
Male
Norway
Rats*
Spleen
Stem Cells
Suspensions
Tibia
Tissue Donors*
Transplants
DNA
Ether
Suspensions