J Korean Pediatr Soc.  2000 May;43(5):644-649.

Typing of Epstein-Barr Virus in Healthy Korean Children and Adults

Affiliations
  • 1Department of Pediatrics, College of Medicine, The Catholic University of Korea.
  • 2Department of Clinical Pathology, College of Medicine, The Catholic University of Korea.

Abstract

PURPOSE: Recently, the list of Epsterin-Barr Virus (EBV)-associated diseases has been growing. It is now known that there are two types (type 1 & 2) of EBV, but it has yet to be fully investigated as to whether type B EBV differs in any way from type A virus in terms of geographical distribution or disease association. We performed this study to find out the prevalence of the two types of EBV in EBV seropositive healthy children and adults by using one step Polymerase chain reaction(PCR).
METHODS
Fifty-four viral capsid antigen (VCA) IgG positive healthy children (5 to 15 yrs of age) and 60 healthy adults (20 to 50 yrs of age) were involved in this study. DNA extracted from saliva samples of healthy study children and adults were subjected to amplification using the modified one step general PCR, which was developed by M. Kunimoto et al for detection of EBV types. And B95-82 cells (type 1) and Jijoye cells (type 2) were used as positive controls, and K-562 cells was used as a negative control.
RESULTS
EBV was detected in mouth washing saliva of 8 (14.8%) of 54 healthy children. Five EBV detected cases contained type 1 and the remaining three cases contained type 2. EBV was detected in mouth washing saliva of 21 (35%) of 60 healthy adults. Twenty EBV detected cases contained type 1 and the remaining one was type 2. In total, EBV was detected in the saliva samples of 29 cases (25.4%), and 25 (86.2%) contained type 1.
CONCLUSION
The results of our study indicate that type 1 is highly dominant in Korea, and EBV excretion is more frequent in EBV positive healthy adults compared to EBV positive healthy children in Korea.

Keyword

Epstein-Barr Virus; Type; Healthy children and adults; PCR

MeSH Terms

Adult*
Capsid
Child*
DNA
Herpesvirus 4, Human*
Humans
Immunoglobulin G
Korea
Mouth
Polymerase Chain Reaction
Prevalence
Saliva
DNA
Immunoglobulin G
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