J Korean Ophthalmol Soc.  2002 Jul;43(7):1276-1286.

Experimental Transscleral Ocular Drug Delivery: Use of Pluronic F-127 Gel and Fibrin Glue as a Sustained Release System

Affiliations
  • 1Department of Ophthalmology, College of Medicine, Yeungnam University, Taegu, Korea. sbummlee@med.yu.ac.kr
  • 2Department of Ophthalmology, School of Medicine, Kyungpook National University, Taegu, Korea.
  • 3Emory Eye Center, Emory University, Atlanta, Georgia, USA.

Abstract

PURPOSE: The purpose of this study was to evaluate the effect of two sustained release systems (Pluronic F-127 gel and Fibrin glue) on the diffusion of dexamethasone across the human sclera.
METHODS
Scleral sections excised from moist-chamber-stored human globes were mounted in a perfusion chamber that can create transscleral pressure. In the sustained release study, sample (100 muL) of 3H-dexamethasone in Pluronic F-127 gel or Fibrin glue was added to the episcleral side of the tissue, while BSS plusR was perfused across the uveal side at an transscleral pressure of 15 mmHg. Perfusate fractions were collected and measured using scintillation spectrometry and scleral permeability was calculated.
RESULTS
The apparent permeability constants of the human sclera to 3H-dexamethasone in BSS plus(R) (the control value), Pluronic F-127 gel, and Fibrin glue were 1.15+/-0.11x10(-5) cm/s (n=5), 1.49+/-0.33x10-6 cm/s (n=5), and 7.32+/-0.98x10(-6) cm/s (n=7), respectively. The permeability values of Pluronic F-127 gel and Fibrin glue were relatively lower than the control value. Pluronic F-127 gel and Fibrin glue showed a uniform sustained release characteristic during a 24-hour period. The cumulative release rates of dexamethasone through the human sclera from BSS plus(R) (the control value), Pluronic F-127 gel, and Fibrin glue were 84.0+/-1.5% (n=5), 29.3+/-5.8% (n=5), and 61.5+/-5.9% (n=4) at 20 hours, respectively. There were significant differences in the human scleral permeability constants and cumulative release rates among the three vehicles (p<0.0001).
CONCLUSIONS
Pluronic F-127 gel and Fibrin glue provided a slow, uniform sustained release during a 24- hour period. This study established a strong possibility of the new transscleral drug delivery in vitro using the sustained release systems of Pluronic F-127 gel and Fibrin glue. This may be a good experimental tool in the future development of a practical drug delivery system across the sclera for the treatment of a variety of chorioretinal disorders.

Keyword

Fibrin glue; Pluronic F-127 gel; Scleral permeability; Sustained release system; Transscleral ocular drug delivery

MeSH Terms

Dexamethasone
Diffusion
Drug Delivery Systems
Fibrin Tissue Adhesive*
Fibrin*
Humans
Perfusion
Permeability
Poloxamer*
Sclera
Spectrum Analysis
Dexamethasone
Fibrin
Fibrin Tissue Adhesive
Poloxamer
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