J Korean Ophthalmol Soc.  1998 Oct;39(10):2292-2297.

MHC Class II Positive Cells and Macrophages in the Iris of normal Lewis Rats

• Han YB

Abstract

To identify the MHC class II expressing cells and macrophages in the iris, immunohistochemistry was carried out using monoclonal antibodies specific to MHC class II antigens(OX6), resident tissue macrophages(ED2) and monocyte-macrophage lineage(ED1) on wholemounts of iris from normal Lewis rats. A number of OX6 positive cells are distributed evenly in the iris. Morphology of OX6 positive cells are dendritiform, round, or pleomorphic. OX6-positive and dendritiform cell are presumed to be dendritic cells. ED2 positive cells are more closely arranged than OX6 positive cells. Most of ED2 positive cells are dendritiform. Most of ED1 positive cells are round. These cells might have important roles in the process of immune mechanism to various antigens in the anterior segment of the eye.

Keyword

Immunohistochemistry; MHC class II; Dendritic cell; Macrophages

MeSH Terms

Animals
Antibodies, Monoclonal
Dendritic Cells
Immunohistochemistry
Iris*
Macrophages*
Rats*
Antibodies, Monoclonal