J Korean Ophthalmol Soc.  1995 Sep;36(9):1498-1502.

The Effect of Intraocular Hypothermia on RPE Cell Proliferation

• Lee SC
• Kwon OW

Abstract

The effects of intraocular hypothermia on experimental proliferative vitreoretinopathy in rabbits were studied. Vitrectomy was performed for 30 minutes with irrigating balanced salt solution in rabbit eyes, and the cultured retinal pigment epithelial cells were injected into the vitreous cavity. The irrigating balanced salt solution was used at room temperature in control eyes, and was cooled to 6 degrees C in experimental eyes. Experimental eyes showed less postoperative inflammation and demonstrated less occurrence of tractional retinal detachment compared with the control eyes. In vitro, the growth of cultured human retinal pigment epithelial ceDs was studied in the conditions of different temperatures. Significant suppression of cell growth was noted at 43 degrees C and also at 4 degrees C. The cooled irrigating solution may be used during vitrectomy procedure to decrease the occurrence of proliferative vitreoretinopathy.

Keyword

Hypothermia; Proliferative vitreoretinopathy; Vitrectomy

MeSH Terms

Cell Proliferation*
Epithelial Cells
Humans
Hypothermia*
Inflammation
Rabbits
Retinal Detachment
Retinaldehyde
Traction
Vitrectomy
Vitreoretinopathy, Proliferative
Retinaldehyde