J Korean Ophthalmol Soc.  1995 Mar;36(3):466-472.

Effects of 5-fluorouracil on culture of Bovine Retinal Pigment Epithelial cells

• Kwak NH
• Yoo JS
• Huh W

Abstract

Inspite of technical advances, long term retinal reattachment of proliferative vitreoretinopathy following the successful surgery is often disturbed by the occurrence of cell proliferation and traction. A variety of antimetabolite or anticancer drugs have been tried to use for testing their antiproliferative effects on proliferative vitreoretinopathy. To evaluate the antiproliferative property of 5-FU, we treated with the different concentration of 5-FU(0.5, 1, 5, 10 mg/L) on cultured bovine retinal pigment epithelial cells and performed dose inhibition studies. We also examined the 5-FU treated bovine retinal pigment epithelial cells by inverted microscope daily to evaluate the morphologic change for 5 days. The results were as follows; 1. 5-FU inhibited proliferation of bovine pigment epithelial cells in a dose dependent and a time dependent manner. 2. The drug concentration required for 50% inhibition of cell growth(ID50) was found to be 1.87 mg/L. 3. As the drug concentration increased, the cell density gradually decreased and the degenerative changes such as irregular shape of cells, cytoplasmic vaculoes and cell debris were aggravated. In 10 mg/L of 5-FU treated well, disruption of cell membrane and cell death were noted from the first day of incubation. These results show that 5-FU has a potent antiprolifertive effects in retinal pigment epithelial cell and toxic effet in high dose.

Keyword

Proliferative vitreoretinopathy; 5-FU; ID50

MeSH Terms

Cell Count
Cell Death
Cell Membrane
Cell Proliferation
Cytoplasm
Epithelial Cells*
Fluorouracil*
Retinaldehyde*
Traction
Vitreoretinopathy, Proliferative
Fluorouracil
Retinaldehyde