J Korean Ophthalmol Soc.
1976 Dec;17(4):415-419.
MIF Test Using Lens Protein as Antigen
- Affiliations
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- 1Department of Ophthalmology, College of Medicine, Seoul National University, Seoul, Korea.
Abstract
- This experiment was performed to demonstrate that the cell-mediated hypersensitivity plays an important role in the development of the so-called endophthalmitis phaooanaphylactica. Fifteen albino guinea pigs were immunized with sclubte proteins of bovine lens with complete Freund's adjuvant. The immunizations were repeated 4 times at 5 days intervals. 5 days after the last injection, the skin test was performed with soluble lens protein, and liquid paraffin was injected into the peritoneal cavity. 7 days after paraffin injection, the peritoneal exudate cells were harvested, and were allowed to migrate on agar with and without antigen according to Salvin- Niahio method. All of them shows positive skin reaction and significant inhibition of migration under the presence of the antigen.
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