J Korean Soc Plast Reconstr Surg.  1998 Oct;25(7):1317-1327.

A Study on expression of e-cadherin during palatal development tn albino rat

• No authors listed

Abstract

As E-cadherin is essential molecule in early developmental process, differentiation and growth of tissue, the investigator has undertaken this study for demonstrating the expression of E-cadyherin during the palatal development in rat. In this study fetuses at the 13th, 14th, 15th, 16th and 18th day of gestation were used as experimental animals. The specimens were stained with hematoxylin and eosin for normal developmental process of the palate, and E-cadherin immunohistochemistry was performed. The results obtained were as follow; 1. At the 13th day of gestation, palatal processes protruded from the maxillary prominence are visible, and the oral cavity has wide communication with the nasal cavity. In E-cadherin immunohistochemistry medial edge epithelium of the palatal process is shown trace reactivity, and epithelium faced with the oral cavity is revealed weakly positive reactivity. 2. At the 14the day of gestataion, palatal processes are elevated above the tongue, and oral cavity is begun to be divided from the nasal cavity by this processes. In E-cadherin immunohistochemistry medial edge epithelium and nasal part epithelium are shown weakly positive reactivity, epithelium faced with the oral cavity is revealed moderately positive reactivity. 3. At the 15th day of gestation, both palatal processes are fused in the midline, and oral cavity is completely divided from the nasal cavity. In E-cadherin immunohistochemistry midline fused epithelium is shown trace reactivity, and epithelium faced with the oral cavity is revealed weakly or moderately positive reactivity. 4. At 16th day of gestation, fusion line is disappeared. In E-cadherin immunohistochemistry palatal epithelium is revealed moderately or strongly positive reactivity. 5. At the 18th day of gestation, mesenchyme in the palate is begun to condense. In E-cadherin immunohistochemistry palatal epithelium is shown strongly positive reactivity. According to the above results at the 13th and 14th day of gestation medial edge epithelium in the tip of palatal process was shown trace or weakly positive reaction, and epithelium faced with oral cavity was revealed weakly or moderately positive reactivity. Palatal epithelium faced with oral cavity was shown moderately or strongly positive reaction from the 15th day to the 18the day of gestation. Consequently it is suggested that the epithelium faced with the oral cavity may play more important role in the process of palate fusion.

MeSH Terms

Animals
Cadherins*
Eosine Yellowish-(YS)
Epithelium
Fetus
Hematoxylin
Humans
Immunohistochemistry
Mesoderm
Mouth
Nasal Cavity
Palate
Pregnancy
Rats*
Research Personnel
Tongue
Cadherins
Eosine Yellowish-(YS)
Hematoxylin