J Lab Med Qual Assur.  2009 Jun;31(1):183-186.

Preparation of Pooled Sera by Using Stirrer

• Park J
• Kim Y

Abstract

BACKGROUND
The aim of this study was to compare a new stir method with the conventional refrozen method used to prepare pooled sera.
METHODS
We prepared fifteen different pooled sera by using two pooled sera preparation methods. Twenty dispensed sera from each pooled serum were analyzed for glucose, total protein, albumin, aspartate aminotransferase, total bilirubin, triglyceride, blood urea nitrogen, creatinine, sodium, potassium, and chloride concentrations. For stability study, six different pooled sera were stored at room temperature, 4degrees C, and -70degrees C for 3 months and the eleven components were measured for each month.
RESULTS
There was no difference in homogeneity between stir and refrozen pooled sera preparation methods and good stability was observed for -70degrees C storage of all measured components.
CONCLUSIONS
The stir method might be more useful than refrozen method to prepare pooled sera.

Keyword

Stir; Pooled sera; Quality control

MeSH Terms

Aspartate Aminotransferases
Bilirubin
Blood Urea Nitrogen
Creatinine
Glucose
Potassium
Quality Control
Sodium
Aspartate Aminotransferases
Bilirubin
Creatinine
Glucose
Potassium
Sodium