J Korean Acad Prosthodont.  2004 Jun;42(3):301-306.

Adsorption of human recombinant fibronectin to titanium in vitro

Affiliations
  • 1Department of Prosthodontics, College of Dentistry, Seoul National University.
  • 2Department of Prosthodontic dentistry, Samsung Medical Center, School of Medicine, Sungkyunkwan University.
  • 3Intellectual Biointerface Engineering Center, Seoul National University College of Dentistry.

Abstract

STATEMENT OF PROBLEM: Fibronectin mediates its biological effects by binding to integrins on cell membranes through a consensus site including the Arg-Gly-Asp (RGD) sequence within tenth type III module. PURPOSE: The purpose of our study was to investigate the adsorption affinity of human recombinant fibronectin peptide (hFNIII 9-10) to titanium and to investigate the effect of the surrounding ionic composition on the adsorption process. MATERIAL AND METHODS: As for evaluating the affinity of hFNIII9-10 to Ti, titanium disks were incubated in 40, 80 and 120 micromgram/ml hFNIII9-10 solution at 37degrees C overnight, repectively. As for evaluating the effect of surrounding ionic concentration, hFNIII9-10 was dissolved in distilled water, phosphate buffered saline and RPMI 1640. Optical density (O.D.) was measured in ELISA reader. RESULTS: The results were as follows; 1. The adsorption of hFNIII 9-10 showed significantly highest mean optical density (O.D.) value in 80 micromgram/ml. 2. The difference of ionic composition in DW, PBS and RPMI did not influence the adsorption amount of hFNIII 9-10.

Keyword

Fibronectin; Titanium

MeSH Terms

Adsorption*
Cell Membrane
Consensus
Enzyme-Linked Immunosorbent Assay
Fibronectins*
Humans*
Integrins
Titanium*
Water
Fibronectins
Integrins
Titanium
Water
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