J Korean Acad Prosthodont.  2014 Jul;52(3):211-221. 10.4047/jkap.2014.52.3.211.

Effects of SLA surface treated with NaOH on surface characteristics and response of osteoblast-like cell

• Park JC
• Kim JH
• Kang ES
• Ryu JJ
• Huh JB

• KMID: 2195430
• DOI: http://doi.org/10.4047/jkap.2014.52.3.211

Abstract

PURPOSE
The purpose of this study was to evaluate the surface characteristics and response of osteoblast-like cell at SLA surface treated with NaOH.
MATERIALS AND METHODS
Three kinds of specimens were fabricated for the experiment groups. Control group was a machined surface, SLA group was a conventionally SLA treated surface, and SLA/NaOH gorup was SLA surface treated with NaOH. To evaluate the surface characteristics, the surface elemental composition (XPS), surface roughness and surface contact angle were evaluated in each group. And the cytotoxicity, cell adhesion, cell proliferation and ATP activity of osteoblast-like cells (MG-63 cells) were compared in each group for evaluatation of the cell responses. Statistical comparisons between groups were carried out via one-way ANOVA using the SPSS software (SPSS Inc., Chicago, USA), and then performed multiple comparisons. The differences were considered statistically significant at P<.05.
RESULTS
SLA surface treated with NaOH (SLA / NaOH group) was changed to hydrophilic surface. All groups did not show the cytotoxicity to the MG-63. In cell adhesion studies, SLA / NaOH group showed the higher degree of adhesion than anothers (P<.05), Up to 7 days of incubation, the proliferation was showed the increasing tendency in all groups but SLA / NaOH group showed the highest cell proliferation between the three groups (P<.05). At 7 days of incubation, there was no difference in ALP activities between the three groups, but at 14 days, SLA / NaOH group showed significant increase in ALP activities (P<.05).
CONCLUSION
In this study, SLA surface treated with NaOH promoted cell adhesion, proliferation and differentiation. It means that SLA/NaOH group is possible to promote osseointegration of implants.

Keyword

SLA surface; NaOH; hydrophilic; MG-63 cell; Cell culture

MeSH Terms

Adenosine Triphosphate
Cell Adhesion
Cell Culture Techniques
Cell Proliferation
Osseointegration
Adenosine Triphosphate

Figure

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  Fig. 1. Non-Contact 3D Video Measuring images of each group. (A) Machined,(B) SLA, (C) SLA/NaOH group.

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  Fig. 2. scanning electron microscope (Hitachi S-4700 SEM, Japan) images of each group (×2000 magnification). (A) Machined, (B) SLA, (C) SLA/NaOH group.

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  Fig. 3. Contact angle of each group. (A) machined group, (B) SLA group, (C) SLA/NaOH group.

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  Fig. 4. Cytotoxicity test of machined group, SLA group and SLA/NaOH group against osteoblasts (MG-63 cells).

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  Fig. 5. Live/dead assay for machined group, SLA group and SLA/NaOH group against osteoblasts (MG-63 cells).

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  Fig. 6. Cells adhesion and spread of MG-63 cells on machined group, SLA group and SLA/NaOH group after 1 day.

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  Fig. 7. Cells adhesion of MG-63 cells on machined group, SLA group and SLA/NaOH group after 3 h.

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  Fig. 8. Cell proliferation of osteoblast-like cells (MG-63 cells) grown on machined group, SLA group and SLA/NaOH group after day 3, and day 7.

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  Fig. 9. ALP activity of osteoblast-like cells (MG-63 cells) cultured on machined group, SLA group and SLA/NaOH group after 7 and 14 day.

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