J Korean Neurosurg Soc.  2010 Jul;48(1):8-13. 10.3340/jkns.2010.48.1.8.

Comparative Analysis of Serum Proteomes of Moyamoya Disease and Normal Controls

Affiliations
  • 1Department of Neurosurgery, Chonbuk National University Medical School, Jeonju, Korea. hayoungc@jbnu.ac.kr
  • 2Department of Pharmacology, Chonbuk National University Medical School, Jeonju, Korea.

Abstract


OBJECTIVE
The etiology and pathogenesis of moyamoya disease remain unclear. Furthermore, the definitive diagnostic protein-biomarkers for moyamoya disease are still unknown. The present study analyzed serum proteomes from normal controls and moyamoya patients to identify novel serological biomarkers for diagnosing moyamoya disease.
METHODS
We compared the two-dimensional electrophoresis patterns of sera from moyamoya disease patients and normal controls and identified the differentially-expressed spots by matrix-assisted laser desorption/ionization-time-of flight mass spectrometry and electrospray ionization quadruple time-of-flight mass spectrometry.
RESULTS
We found and analyzed 22 differently-expressed proteomes. Two proteins were up-regulated. Twenty proteins were down-regulated. Complement C1 inhibitor protein and apolipoprotein C-III showed predominantly changed expressions (complement C1 inhibitor protein averaged a 7.23-fold expression in moyamoya patients as compared to controls, while apolipoprotein C-III averaged a 0.066-fold expression).
CONCLUSION
Although our study had a small sample size, our proteomic data provide serologic clue proteins for understanding moyamoya disease.

Keyword

Moyamoya disease; Proteome

MeSH Terms

Apolipoprotein C-III
Biomarkers
Complement C1 Inhibitor Protein
Electrophoresis
Humans
Mass Spectrometry
Moyamoya Disease
Proteins
Proteome
Sample Size
Apolipoprotein C-III
Complement C1 Inhibitor Protein
Proteins
Proteome
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