J Korean Neurosurg Soc.  1989 Apr;18(4):525-533.

An Experimental Study of the Biochemical Alterations in the Acute Focal Cerebral Ischemia:Disturbance of Energy Metabolism

• Sheen YC
• Shin KM

Abstract

The energy metabolism of the brain has been measured in cat model using high performance liquid chromatography(HPLC). The experimental groups were divided into three according to the duration of ischemia. In 1- and 3-hour ischemia groups, recirculation had increased the ATP, UTP and GTP significantly to 39-49%, 53-57% and 39-62% of the sham control value respectively. Also in these groups, recirculation had increased adenylate energy charge(E.C.) to 75-82% of sham control value. Whereas there were slight increase in adenylate E.C. after recirculation in 5-hr ischemia group, with the remainders not increasing significantly. The Na+, K+-ATPase activities were not significant statistically among the groups. These results suggest that in order to prevent from the irreversible ischemic brain damage, restoration of blood flow must be accomplished within 3 hours from the onset of the acute focal ischemia in cat.

Keyword

Acute focal cerebral ischemia; HPLC; ATP; UTP; GTP; Energy change

MeSH Terms

Adenosine Triphosphate
Animals
Brain
Cats
Chromatography, High Pressure Liquid
Energy Metabolism*
Guanosine Triphosphate
Ischemia
Uridine Triphosphate
Adenosine Triphosphate
Guanosine Triphosphate
Uridine Triphosphate