J Korean Assoc Maxillofac Plast Reconstr Surg.  2005 Mar;27(2):123-130.

Expression of TGF-betaand IGF-I during osseointegration of titanium implant

• Lee IW
• Song HC
• Jee YJ

Abstract

Many of the molecular and genotypic events taking place at the osteoblast cell level during bone-implant integration are still largely unknown. The objective of this study was to examine expression patterns of TGF-beta and IGF-I related genes during bone-implant integration. Titanium implants with machined surface were placed into 8 rabbit tibias. At 3rd, 7th, 14th, 28th day after implantation, the expression pattern of TGF-beta and IGF-I genes in bone with or without implant was examined using reverse transcriptase-polymerase chain reaction (RT-PCR). At the same time, histomorphometric analysis was evaluated, respectively. The bone-to-implant contacts (BIC) of experimental groups were 5.2%, 6.2%, 6.6%, 24.6% at 3rd, 7th, 14th, 28th day. This indicated that newly formed bone increased at the implant surface in bone marrow space after implantation. The expressions of TGF-beta and IGF-I were higher in implantation groups than untreated control groups during all experimental days. The increased expression of TGF-beta and IGF-I genes may be associated with the increased bone-toimplant contact. This result provided the evidence for existing biologic differences in tissue response after implantation and helped us to understand molecular biologic processes in tissue-implant integration.

Keyword

Osseointegration; Titanium implant; TGF-beta; IGF-I; Histomorphometric analysis; RT-PCR analysis

MeSH Terms

Bone Marrow
Insulin-Like Growth Factor I*
Osseointegration*
Osteoblasts
Tibia
Titanium*
Transforming Growth Factor beta
Insulin-Like Growth Factor I
Titanium
Transforming Growth Factor beta