J Gynecol Oncol.  2012 Apr;23(2):115-119. 10.3802/jgo.2012.23.2.115.

Photodynamic therapy for breast cancer in a BALB/c mouse model

• Ahn TG
• Lee BR
• Choi EY
• Kim DW
• Han SJ

• KMID: 2177585
• DOI: http://doi.org/10.3802/jgo.2012.23.2.115

Abstract

OBJECTIVE
Photodynamic therapy (PDT) has been used for superficial neoplasms and its usage has been recently extended to deeper lesions. The purpose of this study was to observe whether or not PDT can cure breast cancer in the solid tumor model, and to define the critical point of laser amount for killing the cancer cells.
METHODS
Twenty four BALB/c mouse models with subcutaneous EMT6 mammary carcinomas were prepared. Mice were divided into eight groups depending on the amount of illumination, and the tumor size was between 8 mm and 10 mm. We began by peritoneal infiltration with a photosensitizer 48 hours prior to applying the laser light, and then we applied a non-thermal laser light. The energy was from 350 J/cm2 to 30 J/cm2 to the cancer.
RESULTS
Regardless of the tumor size from 8 mm to 10 mm, all mice apparently showed positive results via PDT. We also did not find any recurrence over 90 J/cm2. In all models, the color of the breast cancer lesions began to vary to dark on 2 days post PDT and the tumor regression began simultaneously. Also, we confirmed the complete regression of the breast cancer 21 days after PDT.
CONCLUSION
We confirmed that PDT may treat breast cancers that are sized less 10 mm in mouse models. The moderate energy to destruct the breast cancer cells may be 90 J/cm2. Therefore, we can expcect that PDT may be utilized to treat breast cancer, but we need more experience, skills and processing for clinical trials.

Keyword

Breast neoplasms; Inbred BALB C; Mice; Photodynamic therapy

MeSH Terms

Animals
Breast
Breast Neoplasms
Homicide
Light
Lighting
Mice
Photochemotherapy
Recurrence
Triazenes
Triazenes

Figure

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  Fig. 1 Photodynamic therapy illumination onto the cancer with a non-thermal laser light (Ceralas Diode Laser 932 System).

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  Fig. 2 Complete remission was shown in 350 and 240 J/cm2 but tissue deformities were noticed because of high energy (21 days after photodynamic therapy).

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  Fig. 3 Complete remission shown in 180, 150, 120, and 90 J/cm2 (21 days after photodynamic therapy).

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  Fig. 4 The first and third mice showed complete remission but the second one (arrow) revealed no response with 60 J/cm2 (21 days after photodynamic therapy).

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  Fig. 5 All mice showed no response. Cancer growth was continued with 30 J/cm2 (21 days after photodynamic therapy).

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