J Adv Prosthodont.  2014 Feb;6(1):30-34. 10.4047/jap.2014.6.1.30.

Biofilm-forming ability and adherence to poly-(methyl-methacrylate) acrylic resin materials of oral Candida albicans strains isolated from HIV positive subjects

• Uzunoglu E
• Yildirim Bicer AZ
• Dolapci I
• Dogan A

• KMID: 2176567
• DOI: http://doi.org/10.4047/jap.2014.6.1.30

Abstract

PURPOSE
This study evaluated the adhesion to acrylic resin specimens and biofilm formation capability of Candida albicans strains isolated from HIV positive subjects' oral rinse solutions.
MATERIALS AND METHODS
The material tested was a heat-cured acrylic resin (Acron Duo). Using the adhesion and crystal violet assays, 14 oral Candida albicans isolated from HIV-positive subjects and 2 references Candida strains (C. albicans ATCC 90028 and C. albicans ATCC 90128) were compared for their biofilm production and adhesion properties to acrylic surfaces in vitro.
RESULTS
There were no significant differences in adhesion (P=.52) and biofilm formation assays (P=.42) by statistical analysis with Mann-Whitney test.
CONCLUSION
Denture stomatitis and increased prevalence of candidal carriage in HIV infected patients is unlikely to be related to the biofilm formation and adhesion abilities of C. albicans to acrylic resin materials.

Keyword

PMMA; Candida albicans; Biofilms; Adhesion assay

MeSH Terms

Biofilms
Candida albicans*
Candida*
Gentian Violet
HIV*
Humans
Polymethyl Methacrylate
Prevalence
Stomatitis, Denture
Gentian Violet
Polymethyl Methacrylate

Reference

1. Chen SY, Liang WM. Effects of fillers on fiber reinforced acrylic denture base resins. Mid Taiwan J Med. 2004; 9:203–210.

2. Chen SY, Liang WM, Yen PS. Reinforcement of acrylic denture base resin by incorporation of various fibers. J Biomed Mater Res. 2001; 58:203–208.

3. Jagger DC, Harrison A, Jandt KD. The reinforcement of dentures. J Oral Rehabil. 1999; 26:185–194.

4. Katsikas NG, Huggett R, Harrison A, Vowles RW. The effect of esthetic fibers on the flow properties of an acrylic resin denture base material. Dent Mater. 1994; 10:2–5.

5. Kanie T, Fujii K, Arikawa H, Inoue K. Flexural properties and impact strength of denture base polymer reinforced with woven glass fibers. Dent Mater. 2000; 16:150–158.

6. Miettinen VM, Vallittu PK, Docent DT. Water sorption and solubility of glass fiber-reinforced denture polymethyl methacrylate resin. J Prosthet Dent. 1997; 77:531–534.

7. O'Brien WJ. Dental materials and their selection. 4th ed. Chicago: Quintessence Publishing;2008. p. 9.

8. McCabe JF, Walls A. Applied dental materials. Edinburg: Blackwell Science;1998. p. 105.

9. Aleva NA, Birman EG, Afonso W Jr, Chavasco JK, Paula CR, Ribeiro A, Pereira LJ. Erythematous candidosis in patients with complete dentures and HIV+/AIDS. Mycoses. 2007; 50:407–411.

10. Verran J, Maryan CJ. Retention of Candida albicans on acrylic resin and silicone of different surface topography. J Prosthet Dent. 1997; 77:535–539.

11. Ellepola AN, Samaranayake LP. Adhesion of oral Candida albicans isolates to denture acrylic following limited exposure to antifungal agents. Arch Oral Biol. 1998; 43:999–1007.

12. Perezous LF, Flaitz CM, Goldschmidt ME, Engelmeier RL. Colonization of Candida species in denture wearers with emphasis on HIV infection: a literature review. J Prosthet Dent. 2005; 93:288–293.

13. Ramage G, Coco B, Sherry L, Bagg J, Lappin DF. In vitro Candida albicans biofilm induced proteinase activity and SAP8 expression correlates with in vivo denture stomatitis severity. Mycopathologia. 2012; 174:11–19.

14. Ramage G, Tomsett K, Wickes BL, López-Ribot JL, Redding SW. Denture stomatitis: a role for Candida biofilms. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004; 98:53–59.

15. Thein ZM, Samaranayake YH, Samaranayake LP. Characteristics of dual species Candida biofilms on denture acrylic surfaces. Arch Oral Biol. 2007; 52:1200–1208.

16. Chandra J, Kuhn DM, Mukher j, Hoyer LL, McCormick T, Ghannoum MA. Biofilm formation by the fungal pathogen Candida albicans: development, architecture, and drug resistance. J Bacteriol. 2001; 183:5385–5394.

17. Junqueira JC, Fuchs BB, Muhammed M, Coleman JJ, Suleiman JM, Vilela SF, Costa AC, Rasteiro VM, Jorge AO, Mylonakis E. Oral Candida albicans isolates from HIV-positive individuals have similar in vitro biofilm-forming ability and pathogenicity as invasive Candida isolates. BMC Microbiol. 2011; 11:247.

18. Li L, Finnegan MB, Özkan S, Kim Y, Lillehoj PB, Ho CM, Lux R, Mito R, Loewy Z, Shi W. In vitro study of biofilm formation and effectiveness of antimicrobial treatment on various dental material surfaces. Mol Oral Microbiol. 2010; 25:384–390.

19. Redding S, Bhatt B, Rawls HR, Siegel G, Scott K, Lopez-Ribot J. Inhibition of Candida albicans biofilm formation on denture material. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009; 107:669–672.

20. Sweet SP, Cookson S, Challacombe SJ. Candida albicans isolates from HIV-infected and AIDS patients exhibit enhanced adherence to epithelial cells. J Med Microbiol. 1995; 43:452–457.

21. Jin Y, Yip HK, Samaranayake YH, Yau JY, Samaranayake LP. Biofilm-forming ability of Candida albicans is unlikely to contribute to high levels of oral yeast carriage in cases of human immunodeficiency virus infection. J Clin Microbiol. 2003; 41:2961–2967.

22. Kennedy MJ. Adhesion and association mechanisms of Candida albicans. Curr Top Med Mycol. 1988; 2:73–169.

23. Uludamar A, Ozkan YK, Kadir T, Ceyhan I. In vivo efficacy of alkaline peroxide tablets and mouthwashes on Candida albicans in patients with denture stomatitis. J Appl Oral Sci. 2010; 18:291–296.

24. Kuhn DM, Chandra J, Mukherjee PK, Ghannoum MA. Comparison of biofilms formed by Candida albicans and Candida parapsilosis on bioprosthetic surfaces. Infect Immun. 2002; 70:878–888.

25. Djordjevic D, Wiedmann M, McLandsborough LA. Microtiter plate assay for assessment of Listeria monocytogenes biofilm formation. Appl Environ Microbiol. 2002; 68:2950–2958.

26. Samaranayake LP, Fidel PL, Naglik JR, Sweet SP, Teanpaisan R, Coogan MM, Blignaut E, Wanzala P. Fungal infections associated with HIV infection. Oral Dis. 2002; 8:151–160.

27. Ganguly S, Mitchell AP. Mucosal biofilms of Candida albicans. Curr Opin Microbiol. 2011; 14:380–385.

28. Gornitsky M, ParadisI I, Landaverde G, Malo AM, Velly AM. A clinical and microbiological evaluation of denture cleansers for geriatric patients in long-term care institutions. J Can Dent Assoc. 2002; 68:39–45.

29. Karaagaclioglu L, Can G, Yilmaz B, Ayhan N, Semiz O, Levent H. The adherence of Candida albicans to acrylic resin reinforced with different fibers. J Mater Sci Mater Med. 2008; 19:959–963.

30. Waltimo T, Tanner J, Vallittu P, Haapasalo M. Adherence of Candida albicans to the surface of polymethylmethacrylate--E glass fiber composite used in dentures. Int J Prosthodont. 1999; 12:83–86.

31. Morgan TD, Wilson M. The effects of surface roughness and type of denture acrylic on biofilm formation by Streptococcus oralis in a constant depth film fermentor. J Appl Microbiol. 2001; 91:47–53.

32. Zhou L, Tong Z, Wu G, Feng Z, Bai S, Dong Y, Ni L, Zhao Y. Parylene coating hinders Candida albicans adhesion to silicone elastomers and denture bases resin. Arch Oral Biol. 2010; 55:401–409.