J Korean Acad Conserv Dent.  2003 Nov;28(6):435-448. 10.5395/JKACD.2003.28.6.435.

Antibacterial effect of polyphosphate on endodontopathic bacteria

• Shin JH
• Park SJ
• Choi GW

• KMID: 2175618
• DOI: http://doi.org/10.5395/JKACD.2003.28.6.435

Abstract

This study was performed to observe the antibacterial effect of polyphosphate (polyP) with various chain lengths (P3~P75) on virulent, invasive strains of P. gingivalis A7A1-28 and W50, and multidrug resistant E. faecalis ATCC29212. P. gingivalis strains were grown in brain-heart infusion broth (BHI) containing hemin and vitamin K with or without polyP. PolyP was added at the very beginning of the culture or during the exponential growth phase of the culture. Inhibition of the growth of P. gingivalis was determined by measuring the absorbancy at 540nm of the grown cells. Viable cell counts of the culture and release of intracellular nucleotide from P. gingivalis were measured. E. faecalis was grown in plain BHI with antibiotics alone or in combination with polyP(calgon; 0.1~1.0%) and the bacterial absorbancy was measured. The overall results suggest that polyP has a strong antibacterial effect on the growth of the virulent strains of P. gingivalis and the antibacterial activity of polyP seems largely bactericidal, accompanying bacteriolysis in which chelation phenomenon is not involved. Although polyP does not exert antibacterial activity against E. faecalis, it appears to increase antibacterial effect of erythromycin and tetracycline on the bacterium. Therefore, polyP alone or in combination with antibiotics may be developed as a candidate for the agent controlling oral infections including endodontic infection.

Keyword

Endodontopathic bacteria; Polyphosphate; Antibacterial effect; P. gingivalis; E. faecalis; Calgon

MeSH Terms

Anti-Bacterial Agents
Bacteria*
Bacteriolysis
Cell Count
Erythromycin
Hemin
Polyps
Tetracycline
Vitamin K
Anti-Bacterial Agents
Erythromycin
Hemin
Tetracycline
Vitamin K

Figure

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  Fig. 1 Inhibitory effect of polyP with various chain lengths(P3~P75) on the growth of P. gingivalis A7A1-28

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  Fig. 2 Inhibitory effect of polyP with various chain lengths(P3~P75) on the growth of P. gingivalis W50

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  Fig. 3 A change in the growth of P. ginvivalis A7A1-28 with polyP75 added in the early exponential phase

• 

  Fig. 4 A change in the growth of P. ginvivalis W50 with polyP75 added in the early exponential phase

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  Fig. 5 The viable cell counts of P. ginvivalis A7A1-28 in the presence of polyP75

• 

  Fig. 6 The viable cell counts P. ginvivalis W50 in the presence of polyP75

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