J Korean Cancer Assoc.  1998 Oct;30(5):1044-1052.

p16INK4A and p15 INK4B Gene Alterations in Primary Uterine Cervical Carcinoma Tissues and Cervical Carcinoma Cell Lines

• Yoo SW
• Kim JW

Abstract

PURPOSE
Alterations of the p15(INK4B) and p16(INK4A) gene which are separated by 25 kb on chromosome 9p21 have been reported in various tumor derived cell lines and primary tumors, but the role of these genes in cervical cancer is unknown. MATERIAL AND METHOD: To determine the frequency of deletions and point mutations of these genes in human cervical cancer, we examined 57 primary tumors and matched normal tissues, and 3 cervical cancer derived cell lines. All the tumor tissues and cell lines were human papil- INK48 lomavirus (HPV)-positive. Deletions or point mutations of exon 2 of the pl5 gene and exons 1, 2, and 3 of the p16(INK4A) gene were examined by polymerase chain reaction (PCR) and direct sequencing, respectively. RESULT: Our data indicate no evidence for intragenic homozygous deletion or point mutation in the cervical cancer or cervical cancer derived cell lines. INK48 INK4A
CONCLUSION
Deletions or point mutations in the p15 or p16 gene may not be required for the development of HPV-positive cervical cancer or for establishment of cervical cancer cell lines.

Keyword

pl6; pl5; Uterine cervical carcinoma

MeSH Terms

Cell Line*
Cyclin-Dependent Kinase Inhibitor p16
Exons
Genes, p16
Humans
Point Mutation
Polymerase Chain Reaction
Uterine Cervical Neoplasms
Cyclin-Dependent Kinase Inhibitor p16