Diabetes Metab J.  2012 Aug;36(4):275-279. 10.4093/dmj.2012.36.4.275.

Balsamic Vinegar Improves High Fat-Induced Beta Cell Dysfunction via Beta Cell ABCA1

• Seok H
• Lee JY
• Park EM
• Park SE
• Lee JH
• Lim S
• Lee BW
• Kang ES
• Lee HC
• Cha BS

• KMID: 2174422
• DOI: http://doi.org/10.4093/dmj.2012.36.4.275

Abstract

BACKGROUND
The aim of this study was to investigate the effects of balsamic vinegar on beta-cell dysfunction.
METHODS
In this study, 28-week-old Otsuka Long-Evans Tokushima Fatty (OLETF) rats were fed a normal chow diet or a high-fat diet (HFD) and were provided with tap water or dilute balsamic vinegar for 4 weeks. Oral glucose tolerance tests and histopathological analyses were performed thereafter.
RESULTS
In rats fed both the both chow diet and the HFD, the rats given balsamic vinegar showed increased insulin staining in islets compared with tap water administered rats. Balsamic vinegar administration also increased beta-cell ATP-binding cassette transporter subfamily A member 1 (ABCA1) expression in islets and decreased cholesterol levels.
CONCLUSION
These findings provide the first evidence for an anti-diabetic effect of balsamic vinegar through improvement of beta-cell function via increasing beta-cell ABCA1 expression.

Keyword

Balsamic vinegar; Islets; Insulin; ABCA1; Diet, high-fat

MeSH Terms

Acetic Acid
Animals
Cholesterol
Diet
Diet, High-Fat
Glucose Tolerance Test
Insulin
Rats
Water
Acetic Acid
Cholesterol
Insulin
Water

Figure

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  Fig. 1 Effects of balsamic vinegar on plasma glucose levels according to the oral glucose tolerance test (OGTT). (A) The OGTT and (B) glucose area under curve were performed on Otsuka Long-Evans Tokushima Fatty rats administered water or balsamic vinegar at 31 weeks of age after a 24-hour fast. Glucose (2 g/kg) was administered orally. CD, chow diet; HFD, high-fat diet; TW, tap water; BV, balsamic vinegar. aP<0.05 compared with tap water-treated chow diet-fed rats, bP<0.05 compared with tap water-treated high fat diet-fed rats.

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  Fig. 2 Immunohistochemical staining of representative pancreatic islets from water administered Otsuka Long-Evans Tokushima Fatty (OLETF) rats and balsamic vinegar administered OLETF rats (×200). Pancreatic sections were stained with antibodies against insulin. (A) Chow diet-fed rats administered water. (B) Chow diet-fed rats administered balsamic vinegar. (C) High-fat diet-fed rats administered water. (D) High-fat diet-fed rats administered with balsamic vinegar. (E) Insulin area/islet area. Data are mean±standard error of the mean. Scale bars are shown in the panel at 200 µm. CD, chow diet; HFD, high-fat diet; TW, tap water; BV, balsamic vinegar.

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  Fig. 3 Histological sections of the pancreas from water administered Otsuka Long-Evans Tokushima Fatty (OLETF) rats and balsamic vinegar administered OLETF rats (×200). Fluorescence staining was done with filipin. Islets boundaries (i) are indicated with dotted lines. (A) Chow diet-fed rats administered water. (B) Chow diet-fed rats administered balsamic vinegar. (C) High-fat diet-fed rats administered water. (D) High-fat diet-fed rats administered balsamic vinegar. Scale bars are shown in the panel at 500 µm.

• 

  Fig. 4 Immunohistochemical staining of representative pancreatic islets from water-administered Otsuka Long-Evans Tokushima Fatty (OLETF) rats and balsamic vinegar-administered OLETF rats (×200). Pancreatic sections were stained with antibodies against ATP-binding cassette transporter subfamily A member 1 (ABCA1). Islets boundaries (i) are indicated with dotted lines. (A) Chow diet-fed rats administered water. (B) Chow diet-fed rats administered balsamic vinegar. (C) High-fat diet-fed rats administered water. (D) High-fat diet-fed rats administered balsamic vinegar. Scale bars are shown in the panel at 200 µm.

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