Diabetes Metab J.  2013 Aug;37(4):270-277. 10.4093/dmj.2013.37.4.270.

Association of Estrogen Receptor alpha Genes PvuII and XbaI Polymorphisms with Type 2 Diabetes Mellitus in the Inpatient Population of a Hospital in Southern Iran

• Mohammadi
• Pourahmadi M
• Mosalanejad M
• Jamali H
• Ghobadifar MA
• Erfanian S

• KMID: 2174268
• DOI: http://doi.org/10.4093/dmj.2013.37.4.270

Abstract

BACKGROUND
Estrogen plays a fundamental role in the pathogenesis of type 2 diabetes mellitus (T2DM). Very few studies have shown the association between estrogen receptor alpha (ERalpha), PvuII and XbaI gene polymorphisms with T2DM in both men and women. We evaluated the hypothesis that PvuII and XbaI polymorphisms of ERalpha gene may be associated with T2DM in adult.
METHODS
From spring of 2010 to the fall of 2011, a case-control study was performed at clinical centers of Jahrom University of Medical Sciences. We included 174 patients with T2DM including men and women and 174 age, sex, and body mass index frequency-matched health controls. We analyzed the PvuII and XbaI polymorphisms of ERalpha by using the polymerase chain reaction-based restriction fragment length polymorphism method.
RESULTS
No significant differences between demographic characteristics of control and patients groups were observed. Allele frequencies of both PvuII and XbaI polymorphisms were significantly different between patients and control subjects (P=0.014 vs. P=0.002, respectively). When the group was separated into women and men, logistic regression analysis of genotype distribution of PvuII (pp vs. Pp+PP) in both sexes revealed that there was no significant association of PvuII genotype with men (odds ratio [OR], 1.67; confidence interval [CI], 0.86 to 3.28; P=0.89) and women (OR, 0.96; CI, 0.53 to 1.74; P=0.12).
CONCLUSION
PvuII and XbaI polymorphisms in ERalpha are related with T2DM in the inpatient population.

Keyword

Diabetes mellitus, type 2; Estrogen receptor alpha; Genetic; Humans; PvuII; XbaI

MeSH Terms

Body Mass Index
Case-Control Studies
Diabetes Mellitus, Type 2
Estrogen Receptor alpha
Estrogens
Female
Gene Frequency
Genotype
Humans
Inpatients
Iran
Logistic Models
Male
Polymorphism, Restriction Fragment Length
Estrogen Receptor alpha
Estrogens

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