Ann Dermatol.  2009 Feb;21(1):35-38. 10.5021/ad.2009.21.1.35.

Comparison of the Expression Profile of JunB, c-Jun, and S100A8 (Calgranulin A) in Psoriasis Vulgaris and Guttate Psoriasis

• Park CC
• Kim KJ
• Woo SY
• Chun JH
• Lee KH

• KMID: 2172062
• DOI: http://doi.org/10.5021/ad.2009.21.1.35

Abstract

BACKGROUND
Psoriasis is a chronic, inflammatory, immune- mediated skin disease. Recently, several psoriasis-linked genetic loci have been reported; PSORS4 contains S100A8 (calgranulin A), and PSOR6 (19p13) locus harbors JunB (19p13.2). S100A8 is considered to be a marker of inflammation in a variety of diseases. The expression of JunB and c-Jun have been reported to be reduced in psoriatic lesions.
OBJECTIVE
We attempted to assess the role and correlation of S100A8, JunB, and c-Jun in the pathogenesis of guttate psoriasis and psoriasis vulgaris by studying whether any difference of immunohistochemical expression existed.
METHODS
Skin biopsy specimens from patients with psoriasis vulgaris (n=37) and guttate psoriasis (n=17), and a normal skin controls (n=9) were utilized in the study. Formalin-fixed and paraffin-embedded tissue sections were prepared and JunB, c-Jun, and calgranulin A were immunohistochemically stained in order to compare the expression of those three proteins in each group.
RESULTS
Reduced JunB expression was observed in patients with psoriasis vulgaris and guttate psoriasis, as compared to patients in the control group; however, c-Jun expression was reduced only in the psoriasis vulgaris group. The expression of S100A8 increased in the psoriasis groups as compared to the control group. In addition, the expression of S100A8 was different between the psoriasis vulgaris and guttate psoriasis groups; S100A8 was expressed more profoundly in the guttate psoriasis group (p<0.05).
CONCLUSION
Our results indicate that S100A8 contributes to the pathogenesis of guttate psoriasis, and it may be a good target for therapy for guttate psoriasis provoked by microorganisms.

Keyword

c-Jun; Guttate psoriasis; JunB; Psoriasis vulgaris; S100A8 (calgranulin A)

MeSH Terms

Biopsy
Calgranulin A
Genetic Loci
Humans
Inflammation
Proteins
Psoriasis
Skin
Skin Diseases
Calgranulin A
Proteins

Figure

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  Fig. 1 Expression of JunB, c-Jun, and S100A8 on the epidermal layers from psoriasis and control patients.

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  Fig. 2 Quantification of cells expressing JunB, c-Jun, and S100A8 in the epidermis. The pixel area that is stained brown was divided from the hematoxylin-stained area, which was designated as the epidermal layer, in order to compare the level of expression of the three proteins in different tissues from the patients and controls. The dots represent the ratio of each of the tissue sections (*p<0.05, **p<0.01).

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