Ann Dermatol.  2011 Feb;23(1):19-22. 10.5021/ad.2011.23.1.19.

The Expressions of TGF-beta1 and IL-10 in Cultured Fibroblasts after ALA-IPL Photodynamic Treatment

• Byun JY
• Lee GY
• Choi HY
• Myung KB
• Choi YW

• KMID: 2171947
• DOI: http://doi.org/10.5021/ad.2011.23.1.19

Abstract

BACKGROUND
Topical photodynamic therapy (PDT) with 5-aminolevulinic acid (ALA) was originally used for treating superficial skin tumors. The application of PDT to other inflammatory dermatoses like acne vulgaris, psoriasis, granuloma annulare, localized scleroderma and lichen sclerosus has recently been introduced. However, the underlying mechanisms are not well understood. We've previously reported the induction of tumor growth factor (TGF)-beta1 and interleukin (IL)-10 after PDT with ALA and intense pulsed light (IPL) in cultured HaCaT cells.
OBJECTIVE
The purpose of this study was to investigate the expressions of TGF-beta1 and IL-10 in cultured fibroblasts after PDT with using ALA and IPL.
METHODS
Cultured fibroblasts were treated with ALA-IPL PDT (1micromol/L of ALA; 0, 4, 8 and 12 J/cm2 of IPL). The expressions of TGF-beta1 and IL-10 were investigated by reverse transcription-polymerase chain reaction and enzyme linked immunosorbent assay.
RESULTS
TGF-beta1 mRNA and protein were reduced down to 0.52- and 0.63-fold, respectively, after PDT and the IL-10 protein was increased up to 2.74-fold after PDT.
CONCLUSION
The reduction of TGF-beta1 was prominent after PDT and so an antisclerotic effect can be expected after PDT. The induction of IL-10 may contribute to the anti-inflammatory effect, which explains the therapeutic benefit of PDT for inflammatory dermatoses.

Keyword

Aminolevulinic acid; Fibroblasts; IL-10; Photodynamic therapy; TGF-beta1

MeSH Terms

Acne Vulgaris
Aminolevulinic Acid
Fibroblasts
Granuloma Annulare
Interleukin-10
Interleukins
Lichen Sclerosus et Atrophicus
Light
Photochemotherapy
Psoriasis
RNA, Messenger
Scleroderma, Localized
Skin
Skin Diseases
Transforming Growth Factor beta1
Triazenes
Aminolevulinic Acid
Interleukin-10
Interleukins
RNA, Messenger
Transforming Growth Factor beta1
Triazenes

Figure

• 

  Fig. 1 The expressions of tumor growth factor (TGF)-β1 and interleukin (IL)-10 protein after aminolevulinic acid (ALA)-intense pulsed light (IPL) measured by enzyme-linked immunosorbent assay. The data represents the mean±SEM from triplicate determinations of at least two independent experiments for each case. *A significant difference compared with the control that was treated with neither ALA nor IPL (p<0.05).

• 

  Fig. 2 Reverse transcription-polymerase chain reaction analysis of the mRNA expression of tumor growth factor (TGF)-β1 after aminolevulinic acid (ALA)-intense pulsed light (IPL).

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