Anat Cell Biol.  2010 Jun;43(2):110-117. 10.5115/acb.2010.43.2.110.

CDH3/P-Cadherin regulates migration of HuCCT1 cholangiocarcinoma cells

• Baek S
• Lee YW
• Yoon S
• Baek SY
• Kim BS
• Oh SO

• KMID: 2168883
• DOI: http://doi.org/10.5115/acb.2010.43.2.110

Abstract

Intrahepatic cholangiocarcinoma is the second most common subtype of primary hepatobilliary cancer. Despite advances in surgical and medical therapy, its survival rate remains poor. Compared to hepatocellular carcinoma (HCC), the most common liver malignancy, the underlying mechanisms of cholangiocarcinoma carcinogenesis are poorly characterized. P-cadherin (CDH3) is a cadherin super family member. Although CDH3 is frequently over-expressed in cholangiocarcinoma tissues, its roles have never been characterized. To determine the roles of CDH3 in cholangiocarcinoma, we investigated CDH3 function in HuCCT1 cells using specific siRNA. Transfection with CDH3 siRNA did not affect proliferation of HuCCT1 cells. However, cell migration and invasion were significantly reduced when CDH3 was down-regulated. In addition, expressions of several biomarkers for epithelial-mesenchymal transition (EMT) were not changed by CDH3 down-regulation. These results suggest that CDH3 regulates cell migration independent of EMT in cholangiocarcinoma cells.

Keyword

Cholangiocarcinoma; CDH3; migration

MeSH Terms

Biomarkers
Cadherins
Carcinoma, Hepatocellular
Cell Movement
Cholangiocarcinoma
Down-Regulation
Epithelial-Mesenchymal Transition
Humans
Liver
Liver Neoplasms
RNA, Small Interfering
Survival Rate
Transfection
Cholangiocarcinoma
Cadherins
Cholangiocarcinoma
Liver Neoplasms
RNA, Small Interfering