J Bacteriol Virol.  2014 Jun;44(2):197-205. 10.4167/jbv.2014.44.2.197.

The Molecular Epidemiology of Circulating Group A Rotavirus in Gwangju Metropolitan City, Korea: 2008~2012

• Kee HY
• Kim MJ
• Kim SH
• Ha DR
• Kim ES
• Jeong HS
• Chung JK
• Lee BJ

• KMID: 2168695
• DOI: http://doi.org/10.4167/jbv.2014.44.2.197

Abstract

Group A rotaviruses are a major cause of acute gastroenteritis in young children worldwide. For the proper management of rotavirus infections, knowledge of the distribution of G and P genotypes including detection of emerging genotype is crucial. Therefore, the aim of this study is to describe epidemiological changes in rotavirus gastroenteritis in Gwangju metropolitan city, South Korea. Stool samples were collected from 14,314 patients with diarrhea, who visited hospitals in Gwangju from 2008 to 2012. Samples were screened for rotavirus with Enzyme-Linked Immunosorbent Assay (ELISA) method and rotavirus P (VP4), G (VP7) genotypes were determined by reverse-transcription polymerase chain reaction. And we performed nucleotide sequencing analysis. Among a total of 14,314 samples investigated 1,982 samples (13.8%) were ELISA positive. Genotyping of Rotavirus was performed using 526 rotavirus samples. The most prevalent circulating G genotype was G1 (40.5%), followed by G2 (27.6%), G3 (19.4%), G9 (9.7%), G4 (2.5%) and G12 (0.4%). The predominant type of P genotypes was P[8] (69.6%), followed by P[4] (27.8%) and P[6] (2.3%). In this study, 13 G-P combinations were detected. From 2008 to 2010, G1P[8] was the most prevalent, followed by G3P[8]. Whereas, 2011 and 2012, G2P[4] was the most common, followed by G1P[8]. Rotavirus gastroenteritis is a common disease associated with significant morbidity, mortality and economic burden. Ongoing rotavirus surveillance to understand the distribution of G and P genotypes will be critical for the development of effective prevention measurements.

Keyword

Group A rotavirus; VP4; VP7; RT-PCR; Genotype; Gwangju metropolitan city

MeSH Terms

Child
Diarrhea
Enzyme-Linked Immunosorbent Assay
Gastroenteritis
Genotype
Gwangju
Humans
Korea
Molecular Epidemiology*
Mortality
Polymerase Chain Reaction
Rotavirus Infections
Rotavirus*

Figure

• 

  Figure 1. Yearly detection rate of group A rotavirus infection in Gwangju metropolitan city during 2008∼2012. Total of 14,314 samples investigated 1,982 samples (13.8%) were ELISA positive. Yearly detection rate were 13.4%, 14.2%, 14.8%, 18.0%, and 7.2%, respectively.

• 

  Figure 2. The Monthly detection rate of group A rotavirus infections in Gwangju metropolitan city during 2008∼2012. The highest percentages were observed from Jan to April, and percentages showed a marked reduction after May in 2008∼2011.

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  Figure 3. Age distribution of group A rotavirus detected 1,982 patients with rotavirus gastroenteritis in Gwangju metropolitan city between 2008 and 2012. Rotavirus was detected mostly in children 5 years (93.7%), and the peak age incidence was 1∼2 years.

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  Figure 4. Frequencies (%) of group A rotavirus G-genotypes among 526 samples in Gwangju metropolitan city from 2008 to 2012. G typing of the VP7 showed that G1 (40.5%), the most predominant circulating genotype (69.6%), followed by G2 (27.6%), G3 (19.4%), G9 (9.7%).

• 

  Figure 5. Frequencies (%) of group A rotavirus P-genotypes among 526 samples in Gwangju metropolitan city from 2008 to 2012. P typing of the VP4 showed that P[8] was the most predominant circulating genotype (69.6%), followed by P[4] (27.8%), P[6] (2.3%).

• 

  Figure 6. Distribution of group A rotavirus G & P genotypes detected acute gastroenteritis in Gwangju metropolitan city from 2008 to 2012. The most prevalent types in 2008∼2010 was G1P[8], followed by G3P[8]. Whereas, 2011 and 2012, G2P[4] was the most common, followed by G1P[8].

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