J Bacteriol Virol.  2005 Mar;35(1):39-47.

Preparation and Characterization of SDF-1alpha Recombinant Adenovirus for ex vivo Expansion of Hematopoietic Stem Cells

Affiliations
  • 1Department of Biochemistry, Biotechnology Research Institute, College of Natural Science, Chungbuk National University, Cheongju, Chungbuk, 361-763, Korea. yhl4177@chungbuk.ac.kr
  • 2College of Pharmacy, Chungnam National University, Yusong, Daejon, 305-764, Korea.
  • 3Department of Internal Medicine, College of Medicine, Chungnam National University, Yusong, Daejon, 305-764, Korea.
  • 4Research Center for Transgenic Cloned Pigs, Chungnam National University, Yusong, Daejon, 305-764, Korea.

Abstract

Stromal cell-derived factor 1 (SDF-1/CXCL12) is a multifunctional cytokine implicated in normal hematopoiesis. We previously reported that SDF-1alpha enhanced the survival of hematopoietic stem and progenitor cells in synergy with other cytokine such as GM-CSF, steel factor, or thrombopoietin. As adult stem cells are very rare, many investigators are trying to expand hematopoietic stem/progenitor cells in vitro. In this study, we constructed an adenoviral vector and produced high titer of recombinant adenoviruses directing robust expression of SDF-1alpha determined by ELISA. We also produced control empty adenoviruses and recombinant LacZ adenoviruses. In order to check the feasibility of SDF-1alpha in ex vivo expansion system, we compared HUVEC cells tranduced by a SDF-1alpha recombinant virus with HUVEC cells transduced by a LacZ recombinant virus in supporting activity of hematopoietic cells, and found that expression of SDF-1alpha in HUVEC cells increased viable blood cell population obtained from the same number of CD34+ cells. The SDF-1alpha recombinant adenovirus seems to be useful for future application in hematopoiesis studies.

Keyword

SDF-1; Adenovirus; ex vivo expansion; HUVEC

MeSH Terms

Adenoviridae*
Adult Stem Cells
Blood Cells
Chemokine CXCL12*
Enzyme-Linked Immunosorbent Assay
Granulocyte-Macrophage Colony-Stimulating Factor
Hematopoiesis
Hematopoietic Stem Cells*
Human Umbilical Vein Endothelial Cells
Humans
Research Personnel
Stem Cell Factor
Stem Cells
Thrombopoietin
Chemokine CXCL12
Granulocyte-Macrophage Colony-Stimulating Factor
Stem Cell Factor
Thrombopoietin
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