Genomics Inform.
2007 Mar;5(1):6-9.
Construction of an RNase P Ribozyme Library System for Functional Genomics Applications
- Affiliations
-
- 1Department of Chemistry and BK School of Molecular Science, Pohang University of Science and Technology, Pohang 790-784, Korea. dlee@postech.edu
- 2Department of Chemistry, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.
Abstract
- An RNase P ribozyme library has been developed as a tool for functional genomics studies. Each clone of this library contains a random 18-mer and the sequence of M1 RNA, the catalytic subunit of RNase P. Repression of target gene expression is thus achieved by the complementary binding of mRNA to the random guide sequence and the successive target cleavage via M1 RNA. Cellular expression of the ribozyme expression was confirmed, and EGFP mRNA was used as a model to demonstrate that the RNase P ribozyme expression system can inhibit the target gene expression. The constructed RNase P ribozyme library has a complexity of 1.4x10(7). This novel library system should become a useful in functional genomics, to identify novel gene functions in mammalian cells.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- An RNA Mapping Strategy to Identify Ribozyme-Accessible Sites on the Catalytic Subunit of Mouse Telomerase
- Construction of Ribozyme Aimed at Hantaan Virus S Genome Segment and Demonstration of Function as Anti-viral Drug
- Specificity of Intracellular Trans-Splicing Reaction by hTERT-Targeting Group I Intron
- RNA Mapping of Mutant Myotonic Dystrophy Protein Kinase 3'-Untranslated Region Transcripts
- Functional Modification of a Specific RNA with Targeted Trans-Splicing
