J Dent Rehabil Appl Sci.  2016 Mar;32(1):16-23. 10.14368/jdras.2016.32.1.16.

Effect of fluoride-containing gel on the roughness of a titanium surface and the promotion of bacterial growth

• Kim SJ
• Lee JK
• Chang BS
• Lee SY
• Um HS

• KMID: 2162375
• DOI: http://doi.org/10.14368/jdras.2016.32.1.16

Abstract

PURPOSE
The aim of this study was to evaluate whether fluorides at various pH cause changes in the surface roughness of titanium implants that alter the adherence of bacterial biofilms.
MATERIALS AND METHODS
The titanium disks were assigned randomly to the following seven groups according to the fluoride agents and application time (1 minute or 30 minute) used: control (no treatment); group 1 (1.23% acidulated phosphate fluoride [APF] at pH 3.5 for 1 minute); group 2 (1.23% APF at pH 3.5 for 30 minute); group 3 (1.23% APF at pH 4.0 for 1 minute); group 4 (1.23% APF at pH 4.0 for 30 minute); group 5 (2% NaF gel at pH 7.0 for 1 minute); group 6 (2% NaF gel at pH 7.0 for 30 minute). The surface roughness of the titanium disks and the amount of adherent bacteria were measured.
RESULTS
Group 2 showed a significantly greater surface roughness than the control group (P < 0.0001). No significant differences in the amount of surface bacteria were observed between the treated samples and the controls. In addition, there were no significant differences in bacterial adherence relative to the incubation period between the treated samples and the controls.
CONCLUSION
The surface roughness of the titanium disks was significantly greater after treatment with APF at pH 3.5 for 30 min compared with that of the controls. In addition, we found that the amount of Porphyromonas gingivalis, Fusobacterium nucleatum, and Aggregatibactor actinomycetemcomitans was similar among all groups.

Keyword

fluorides; titanium; biofilms; bacterial adhesion

MeSH Terms

Acidulated Phosphate Fluoride
Bacteria
Bacterial Adhesion
Biofilms
Fluorides
Fusobacterium nucleatum
Hydrogen-Ion Concentration
Porphyromonas gingivalis
Titanium*
Acidulated Phosphate Fluoride
Fluorides
Titanium

Figure

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  Fig. 1 Percentage of bacterial protein content on differently treated titanium samples relative to the amount measured on the control samples. The protein content measurements showed no significant differences in the amount of bacteria on the treated samples compared with that of the controls.

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  Fig. 2 SEM images of P. gingivalis on the titanium surface (Magnification: ×10,000). SEM images showed no substantial difference among the 4 groups.

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  Fig. 3 SEM images of F. nucleatum on the titanium surface (Magnification: ×10,000). SEM images showed no substantial difference among the 4 groups.

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  Fig. 4 SEM images of A. actinomycetemcomitans on the titanium surface (Magnification: ×10,000). SEM images showed no substantial difference among the 4 groups.

Reference

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