Yonsei Med J.  1996 Aug;37(4):251-261. 10.3349/ymj.1996.37.4.251.

Changes in intracellular Ca2+ concentration of rabbit coronary artery smooth muscle cell during ischemic cardioplegic period

• Lee YH
• Choi GB
• Ahn DS
• Kang BS

• KMID: 2158054
• DOI: http://doi.org/10.3349/ymj.1996.37.4.251

Abstract

To elucidate the possibility whether an elevation of intracellular Ca2+ concentration ([Ca2+]i) in rabbit coronary artery myocytes during ischemic cardioplegic period may serve as one of the mechanisms of the "no-reflow' phenomenon or not, the changes in [Ca2+]i were measured under ischemic cardioplegia conditions using a fluorescent Ca2+ indicator, fura 2/AM. When single cells were perfused with cardioplegic or ischemic cardioplegic solutions, [Ca2+]i was significantly increased and the degree of [Ca2+] elevation was further augmented by the ischemic cardioplegic solution. Pretreatment of a sarcoplasmic reticulum emptying agent, 20 mM caffeine, had no effect on ischemic cardioplegia-induced [Ca2+]i changes, but application of a Ca2+ channel blocker, 5 x 10 (-1)M nifedipine, or an antagonist of Na+/Ca2+ exchange, 5 mM Ni2+, significantly inhibited the [Ca2+]i elevation, respectively. The magnitude of ischemic cardioplegia-induced [Ca2+]i elevation was dependent on the Ca2+ concentration of perfusate in the range of 0 and 25 mM. When Ni2+ was added to the reperfusion solution, recovery of ischemic cardioplegia-induced [Ca2+]i elevation was very rapid compared with the controls. It is concluded that ischemic cardioplegia-induced [Ca2+]i elevation may serve as one of the mechanisms of the "no-reflow' phenomenon in rabbit coronary artery smooth muscle cells. We propose that Na+/Ca2+ exchange may serve as a key function in ischemic cardioplegia-induced [Ca2+]i elevation.

Keyword

Ischemic cardioplegia; 'no-reflow' phenomenon. [Ca2+]i; coronary artery

MeSH Terms

Animal
Arteries/metabolism
Calcium/*metabolism
Coronary Vessels/*metabolism
Female
*Heart Arrest, Induced
Intracellular Membranes/*metabolism
Male
Muscle, Smooth, Vascular/*metabolism/pathology
Myocardial Ischemia/*metabolism/pathology
Osmolar Concentration
Rabbits
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