Ann Rehabil Med.  2016 Feb;40(1):102-110. 10.5535/arm.2016.40.1.102.

Polymorphism of Nitric Oxide Synthase 1 Affects the Clinical Phenotypes of Ischemic Stroke in Korean Population

• Yoo SD
• Park JS
• Yun DH
• Kim HS
• Kim SK
• Kim DH
• Chon J
• Je G
• Kim YS
• Chung JH
• Chung SJ
• Yeo JA

• KMID: 2155172
• DOI: http://doi.org/10.5535/arm.2016.40.1.102

Abstract

OBJECTIVE
To investigate whether four single nucleotide polymorphisms (SNPs) rs2293054 [Ile734Ile], rs1047735 [His902His], rs2293044 [Val1353Val], rs2682826 (3'UTR) of nitric oxide synthase 1 (NOS1) are associated with the development and clinical phenotypes of ischemic stroke.
METHODS
We enrolled 120 ischemic stroke patients and 314 control subjects. Ischemic stroke patients were divided into subgroups according to the scores of the National Institutes of Health Stroke Survey (NIHSS, <6 and ≥6) and Modified Barthel Index (MBI, <60 and ≥60). SNPStats, SNPAnalyzer, and HelixTree programs were used to calculate odds ratios (ORs), 95% confidence intervals (CIs), and p-values. Multiple logistic regression models were performed to analyze genetic data.
RESULTS
No SNPs of the NOS1 gene were found to be associated with ischemic stroke. However, in an analysis of clinical phenotypes, we found that rs2293054 was associated with the NIHSS scores of ischemic stroke patients in codominant (p=0.019), dominant (p=0.007), overdominant (p=0.033), and log-additive (p=0.0048) models. Also, rs2682826 revealed a significant association in the recessive model (p=0.034). In allele frequency analysis, we also found that the T alleles of rs2293054 were associated with lower NIHSS scores (p=0.007). Respectively, rs2293054 had a significant association in the MBI scores of ischemic stroke in codominant (p=0.038), dominant (p=0.031), overdominant (p=0.045), and log-additive (p=0.04) models.
CONCLUSION
These results suggest that NOS1 may be related to the clinical phenotypes of ischemic stroke in Korean population.

Keyword

Nitric oxide synthase 1; Polymorphism; Ischemic stroke; National Institutes of Health Stroke Survey; Modified Barthel Index

MeSH Terms

Alleles
Gene Frequency
Humans
Logistic Models
National Institutes of Health (U.S.)
Nitric Oxide Synthase*
Nitric Oxide*
Odds Ratio
Phenotype*
Polymorphism, Single Nucleotide
Stroke*
Nitric Oxide
Nitric Oxide Synthase

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