Immune Netw.  2007 Dec;7(4):186-196. 10.4110/in.2007.7.4.186.

Enhancement of Antigen-specific Antibody and CD8(+) T Cell Responses by Codelivery of IL-12-encapsulated Microspheres in Protein and Peptide Vaccination

• Park SH
• Chang J
• Yang SH
• Kim HJ
• Kwak HH
• Kim BM
• Lee SH

• KMID: 2150636
• DOI: http://doi.org/10.4110/in.2007.7.4.186

Abstract

BACKGROUND: Although IL-12 has been widely accepted to play a central role in the control of pathogen infection, the use of recombinant IL-12 (rIL-12) as a vaccine adjuvant has been known to be ineffective because of its rapid clearance in the body.
METHODS
To investigate the effect of sustained release of IL-12 in vivo in the peptide and protein vaccination models, rIL-12 was encapsulated into poly (DL-lactic-co-glycolic acid) (PLGA).
RESULTS
We found that codelivery of IL-12-encapsulated microspheres (IL-12EM) could dramatically increase not only antibody responses, but also antigen-specific CD4(+) and CD8(+) T cell responses. Enhanced immune responses were shown to be correlated with protective immunity against influenza and respiratory syncytial virus (RSV) virus challenge. Interestingly, the enhancement of CD8(+) T cell response was not detectable when CD4(+) T cell knockout mice were subjected to vaccination, indicating that the enhancement of the CD8(+) T cell response by IL-12EM is dependent on CD4(+) T cell "help".
CONCLUSION
Thus, IL-12EM could be applied as an adjuvant of protein and peptide vaccines to enhance protective immunity against virus infection.

Keyword

Interleukin 12; PLGA microspheres; vaccine adjuvant; CTL response

MeSH Terms

Animals
Antibody Formation
Influenza, Human
Interleukin-12
Mice
Mice, Knockout
Microspheres*
Respiratory Syncytial Viruses
Vaccination*
Vaccines, Subunit
Interleukin-12
Vaccines, Subunit