J Bacteriol Virol.  2015 Jun;45(2):132-137. 10.4167/jbv.2015.45.2.132.

Molecular Epidemiology of Mumps Virus Circulated in Gwangju

• Kim SH
• Seo M
• Park JW
• Cho SJ
• Ha YD
• Ha DR
• Kim ES
• Kang HJ
• Kim SS
• Kim K
• Chung JK

• KMID: 2149213
• DOI: http://doi.org/10.4167/jbv.2015.45.2.132

Abstract

The recent mumps epidemic in South Korea has generated a large amount of public concern. This study has attempted to analyze molecular epidemiological changes of mumps virus circulating in Gwangju metropolitan area, South Korea. 953 throat swab samples were collected from patients with parotitis from May 2013 to July 2014. The majority (71.5%) of these cases have occurred in middle or high school students aged from 15 to 19 years. All samples were tested using a reverse transcription polymerase chain reaction (RT-PCR) that targets the short hydrophobic (SH) gene of the virus. Mumps virus SH gene was detected in 39.2% (374/953) of samples. And 82 RT-PCR products were randomly selected for nucleotide sequencing analysis. All of these sequences were determined as genotype I by phylogenetic analysis and showed the highest nucleic acid similarity (99%) with Dg1062/Korea/98 (GenBank accession no. AY309060). These results suggested that appearance of new genotype or genetic variation at the nucleotide level could be ruled out to evaluate main cause of recent mumps outbreak in Gwangju metropolitan area.

Keyword

Mumps; Parotitis; Short hydrophobic gene; Genotype I

MeSH Terms

Genetic Variation
Genotype
Gwangju
Humans
Korea
Molecular Epidemiology*
Mumps
Mumps virus*
Parotitis
Pharynx
Polymerase Chain Reaction
Reverse Transcription

Figure

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  Figure 1. Number of mumps suspected cases and RT-PCR positive cases by monthly in an outbreak in Gwangju city, May 2013-July 2014 (n=953).

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  Figure 2. Number of mumps RT-PCR positive cases by age and sex in an outbreak in Gwangju city, May 2013-July 2014 (n=374).

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  Figure 3. Phylogenetic tree of selected mumps virus strains in the study and in GenBank. The tree is based on SH gene sequence alignment (206 bp). Strains detected in the study are marked with full dots (●). Capital letters denote mumps virus genotype.

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