Allergy Asthma Immunol Res.  2015 Sep;7(5):483-488. 10.4168/aair.2015.7.5.483.

Profiles of IgE Sensitization to Der f 1, Der f 2, Der f 6, Der f 8, Der f 10, and Der f 20 in Korean House Dust Mite Allergy Patients

• Jeong KY
• Lee JY
• Son M
• Yi MH
• Yong TS
• Shin JU
• Lee KH
• Kim YJ
• Park KH
• Park HJ
• Lee JH
• Park JW

• KMID: 2147957
• DOI: http://doi.org/10.4168/aair.2015.7.5.483

Abstract

PURPOSE
Measurement of IgE specific to purified house dust mite (HDM) allergens may improve allergy diagnosis. This study aimed to investigate the sensitization profiles of Korean HDM allergic subjects suffering from respiratory allergy and atopic dermatitis (AD) to Der f 1, Der f 2, Der f 6, Der f 8, Der f 10, and Der f 20.
METHODS
Recombinant HDM allergens were produced in Pichia pastoris (Der f 1) or Escherichia coli (5 allergens). IgE reactivity to the individual recombinant allergens and total extract of mite was assessed by ELISA.
RESULTS
Der f 1 was recognized by 79.1%, Der f 2 by 79.1%, Der f 6 by 9.3%, Der f 8 by 6.2%, Der f 10 by 6.2%, and Der f 20 by 6.6% of the patients' sera tested, while the prevalence of IgE reactivity to total mite extract was 94.7%. Combination of Der f 1 and Der f 2 had a sensitivity of 87.6%. Specific IgE to Der f 2 alone was detected from 89.4% of HDM-sensitized respiratory allergy subjects and 92.3% to the combination of the 2 major allergens Der f 1 and Der f 2. However, sera from fewer patients with AD, namely 72.4% and 71.0%, recognized Der f 1 and Der f 2, respectively. The combination of 2 major allergens allowed diagnosis of 84.5% of the AD patients. No correlation between sensitization to specific allergens and HDM allergy entity was found.
CONCLUSIONS
Der f 2 was the most frequently sensitized allergen among the HDM-sensitized respiratory and AD patients in Korea, and the combination of the group 1 and 2 major allergens increased the diagnostic sensitivity. Minor allergens did not significantly improve diagnostic sensitivity. However, further studies are needed to analyze the relationship between sensitization to other HDM allergens and the disease entity of the HDM allergy.

Keyword

Allergen; Component-resolved diagnosis; Dermatophagoides; House dust mites

MeSH Terms

Allergens
Dermatitis, Atopic
Diagnosis
Enzyme-Linked Immunosorbent Assay
Escherichia coli
Humans
Hypersensitivity*
Immunoglobulin E*
Korea
Mites
Pichia
Prevalence
Pyroglyphidae*
Allergens
Immunoglobulin E

Figure

• 

  Fig. 1 Patient groups with HDM allergies. Respiratory allergy group consisted of subjects diagnosed with asthma (AS), allergic rhinitis (AR), and/or allergic conjunctivitis (AC), while the skin allergy group consisted of those diagnosed with atopic dermatitis (AD).

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  Fig. 2 Production of recombinant HDM allergens. Purified each allergen (10 µg) were separated on a 15% polyacrylamide gel containing sodium dodecyl sulfate under reducing conditions. M, molecular mass standard; 01, recombinant Der f 1; 02, recombinant Der f 2; 06, recombinant Der f 6; 08, recombinant Der f 8; 10, recombinant Der f 10; 20, recombinant Der f 20.

• 

  Fig. 3 IgE reactivities of recombinant allergens. Absorbance range (A) and positive rate (B) to each allergen are plotted. The bottom and top edges of the box indicate the intra-quartile range (25th and 75th percentiles, respectively). The horizontal line inside the box indicates the median value. Whiskers denote the 10th and 90th percentiles.

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