J Lab Med Qual Assur.  2011 Dec;33(2):103-109.

Comparison of Two Automated Immunoassays for the Detection of Anti-Hepatitis A Virus Total Immunoglobulin and IgM

• Shin SY
• Lim HJ
• Yi C
• Yu S
• Kwon MJ
• Park H
• Kim YJ
• Jung CL
• Woo HY

Abstract

BACKGROUND
The detection of total anti-hepatitis A virus (anti-HAV) immunoglobulin (Ig) and IgM is important for diagnosing acute hepatitis A. Our laboratory introduced new commercial automated chemiluminescence immunoassays (CLIAs) for use in addition to pre-existing automated CLIA. We evaluated the rate of agreement in the detection of total anti-HAV Ig and IgM in serum samples between two automated CLIAs.
METHODS
We analyzed 181 samples those were submitted for testing at Kangbuk Samsung Medical Center. We analyzed the rate of agreement between the ADVIA Centaur XP (Siemens, Germany) and the MODULAR ANALYTICS E170 (Roche, Switzerland) analyzers. We performed reverse transcription (RT)-PCR when there was a discrepancy between the results from the two analyzers.
RESULTS
The agreement rates between the ADVIA Centaur XP and the MODULAR ANALYTICS E170 for total anti-HAV Ig and IgM were 97.2% and 98.9%, respectively. Discrepant results were obtained in seven cases; all were found to be HAV-negative based on RT-PCR analysis.
CONCLUSIONS
The total anti-HAV Ig and IgM results obtained using the two automated analyzers were comparable. However, in cases of equivocal results tested by the ADVIA Centaur XP for anti-HAV IgM, retesting and follow-up testing of samples are recommended.

Keyword

Hepatitis A virus; Immunoglobulins; ADVIA Centaur XP; MODULAR ANALYTICS E170

MeSH Terms

Hepatitis A
Hepatitis A Antibodies
Hepatitis A virus
Immunoassay
Immunoglobulin M
Immunoglobulins
Luminescence
Reverse Transcription
Viruses
Hepatitis A Antibodies
Immunoglobulin M
Immunoglobulins