Influences of Betadine and Hydrogen Peroxide on Vasospasm

Kim, Hyun Peel; Han, Seung Kyu; Woo, Hong Suh; Kim, Woo Kyung

Abstract

Vasospasm influences on final results of microanastomosis such as free flap or replantation. Postoperative vasospasm was induced by multiple factors. Many patients had to be exposed to repetitive use of dressing materials (Betadine, Hydrogen peroxide), because areas that distal to the microanastomotic sites were vulnerable to infection. Spastic abilities of Betadine and Hydrogen peroxide(H2O2) had not been known. We performed experimental study for influences of Betadine and H2O2 on vasospasm after microanastomosis. Twenty Sprague-Dawley rats(300 - 345g) were randomly assigned to one of two groups, one was Betadine(n = 10) and the other was H2O2(n = 10). The femoral arteries were exposed, bilaterally. The left femoral artery was used as a test site(Betadine, H2O2), and the right as a control (normal saline). The initial arterial diameter were measured under a microscopy using vernier calipers, 20 minutes after dissection. Another 20minutes later, Betadine and H2O2, normal saline were dropped. The final arterial diameter were measured, 10 minutes after dropping. Gross examination was done during 10minutes, too. In Group I, the initial mean arterial diameters were 0.775+/-0.075 mm(Lt.), 0.785 +/- 0.041 mm(Rt.) and the final mean arterial diameters were 0.785 +/- 0.068 mm(Lt), 0.785 +/- 0.048 mm(Rt.). In Group II, the initial mean arterial diameters were 0.775 +/- 0.052 mm(Lt.), 0.780 +/- 0.040 mm (Rt.) and the final mean arterial diameters were 0.765 +/- 0.055 mm(Lt.), 0.775 +/- 0.045 mm(Rt.). There was no significant statistical difference between each materials and its control, and no changes on gross examination.We concluded that there is no influences of Betadine and H2O2 on vasospasm after microanastomosis.