Korean Lepr Bull.
2001 Jun;34(1):47-56.
Optimization for cell lysate preparation of M. leprae from infected nude mouse
- Affiliations
-
- 1nstitute of Hansen's Disease, Department of Pathology College of Medicine, The Catholic University of Korea, Korea.
Abstract
- The method of cell lysate preparation of M. leprae is an important technique in the study of leprosy. This report describes the optimization of method for cell lysate preparation of M. leprae obtained from infected nude mouse. After M. leprae isolated from nude mouse foot-pad was disrupted by sonication, it was centrifuged and then whole lysate was prepared. With this method it was possible to isolate 0.3 mg whole cell lysate using 20 mg of M. leprae. By SDS-PAGE and Coomassie blue staining, the number of protein in M. leprae is less than that of other bacteria, for example, E. coli and M. smegmatis. It is likely that this is due to the small genome size. This work will contribute to the analysis of new protein antigen of M. leprae and the basic study for the development of vaccine in leprosy.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Improved protocols for the purification and maintenance of Mycobacterium lepraestrains in athymic nude mice
- CD11b+Gr-1low cells that accumulate in M.leprae-induced granulomas of the footpad skin of nude mice have the characteristics of monocytic-myeloid-derived suppressor cells
- Identification of regulatory sequence within the promoter of M.leprae-specific 18-kDa gene and its binding factor(s)
- Expression of Nucleotide-oligomerization Domain (NOD) and Related Genes in Mouse Tissues Infected with Mycobacterium leprae
- Mycobacterium Leprae in Cultured Mouse Peritoneal Macrophages: In vivo Infection In vitro Cultivation
