Yonsei Med J.  2014 Jul;55(4):1130-1137. 10.3349/ymj.2014.55.4.1130.

Effects of Transplantation with Marrow-Derived Mesenchymal Stem Cells Modified with Survivin on Renal Ischemia-Reperfusion Injury in Mice

Affiliations
  • 1Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. kyl361@yeah.net

Abstract

PURPOSE
To determine whether renal injury induced by ischemia-reperfusion (I/R) could be further improved by mesenchymal stem cells (MSCs) modified with survivin.
MATERIALS AND METHODS
Lentiviral vectors were used to introduce the survivin gene into MSCs and the MSCs modified with survivin were transplanted into established mice models of renal I/R injury. Seven days later, serum creatinine (Scr) and blood urea nitrogen (BUN) were measured and the survival of MSCs was determined. Hematoxylin and eosin staining was used to assess renal pathological change. The expressions of hepatocyte growth factor (HGF) and basic fibroblast growth factor (bFGF) in kidney tissue were detected by western blot.
RESULTS
Mice transplanted with survivin-modified MSCs demonstrated good renal function recovery with Scr and BUN decline close to normal levels and improvement of renal I/R injury repair. Additionally, the survival of transplanted MSCs modified with survivin was enhanced and the expression of HGF and bFGF in kidney tissue was increased.
CONCLUSION
Our results demonstrated that MSCs engineered to over-express survivin could enhance their therapeutic effect on renal I/R injury in mice, probably via the improved survival ability of MSCs and increased production of protective cytokines in ischemic tissue.

Keyword

Marrow-derived mesenchymal stem cells; ischemia-reperfusion; survivin; transplantation

MeSH Terms

Animals
Bone Marrow Cells/*cytology
Inhibitor of Apoptosis Proteins/*therapeutic use
Male
Mesenchymal Stem Cell Transplantation/*methods
Mice
Mice, Inbred C57BL
Reperfusion Injury/drug therapy/*therapy
Repressor Proteins/*therapeutic use
Inhibitor of Apoptosis Proteins
Repressor Proteins

Figure

  • Fig. 1 Phenotypic characterization of MSCs. (A) The initial passage MSCs grew as a morphologically homogeneous population of fibroblast-like cells. (B) Third passage MSCs grew as whorls of densely packed spindle-shaped. (C-G) Flow cytometry analyzed the surface markers CD29, CD44, CD90, CD34, and CD45 in MSCs. MSC, mesenchymal stem cell.

  • Fig. 2 Efficiency of gene transduction and survivin expression. (A) Expression of EGFP in mock lentivirus-transduced MSCs. (B) Expression of EGFP in MSCs modified with survivin recombinant lentivirus. (C) Survivin expression in MSCs was detected by western blot. MSCs were infected with survivin recombinant lentivirus and mock lentivirus. Total cell lysates were harvested and the presence of survivin protein was detected by specific antibody. β-actin was used as a loading control. MSC, mesenchymal stem cell; EGFP, enhanced green fluorescent protein.

  • Fig. 3 Protective effect of survivin-MSCs on renal function. At 7 days after MSCs transplantation, BUN (A) and Scr (B) measured by Auto Analyzer with BUN and Scr test kits. Scr, serum creatinine; BUN, blood urea nitrogen; MSC, mesenchymal stem cell; I/R, ischemia-reperfusion. *,†p<0.05 versus control group.

  • Fig. 4 Survivin-MSCs attenuated renal injury. (A) Kidney tissue specimens were collected for H&E staining ×200. (B) The renal injury was semi-quantitatively scored. Data are presented as the mean±SD. *p<0.05, compared with control group. †p<0.05, compared with control group. H&E, hematoxylin and eosin, MSC, mesenchymal stem cell; I/R, ischemia-reperfusion.

  • Fig. 5 Survival of transplanted MSCs and HGF and bFGF expression in kidney tissue. (A) Kidney tissue specimens were collected to observe EGFP expression in renal slices by fluorescence microscope. EGFP-positive cells (yellow arrows). (B) Quantitative analysis of the number of survival MSCs at 7 days after transplantation. Data are presented as the mean±SD. *p<0.05, compared with control group. †p<0.05, compared with control group. (C) Kidney tissues were collected and lysed. Tissue lysates were prepared and subjected to western blot. Specific antibodies were used to detect HGF and bFGF. β-actin was used as a loading control. MSC, mesenchymal stem cell; HGF, hepatocyte growth factor; bFGF, basic fibroblast growth factor; EGFP, enhanced green fluorescent protein; I/R, ischemia-reperfusion.


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