J Korean Ophthalmol Soc.  2002 Nov;43(11):2329-2334.

Evaluation of Indocyanine Green Influence on Cultured an Retinal Pigment Epithelial Cells

• Seo JS
• Choi GJ

Abstract

PURPOSE: To evaluate how much the indocyanine green (ICG) influence cultured human retinal pigment epithelial (RPE) cells(ARPE-19; ATCC No. CRL-2302).
METHODS
Human RPE cells were incubated with media and various concentration of ICG. Their viability upon different ICG concentration and exposure time were examined by MTT assay. Also, the amount of ICG to be influxed onto the cells was estimated with the flow cytometry and the confocal laser scanning microscopy.
RESULTS
The viability examined by MTT assay decreased upon different ICG concentration and exposure time. Also, they were effected in the condition of very low dose of ICG (0.00016%, 0.0008%). The amount of ICG to be influxed onto cells increased upon its concentration and time to be exposed.
CONCLUSIONS
In the exposure of RPE cells to ICG, it accumulated in the cells and their viability decrease. These results should be taken into account when ICG is used for macular hole surgery.

Keyword

Indocyanine green; MTT assay; Retinal pigment epithelium

MeSH Terms

Epithelial Cells*
Flow Cytometry
Humans
Indocyanine Green*
Microscopy, Confocal
Retinal Perforations
Retinal Pigment Epithelium
Retinaldehyde*
Indocyanine Green
Retinaldehyde