J Korean Ophthalmol Soc.  1997 Jan;38(1):86-93.

Comparison of the Retinal Microvasculature between Normal and Diabetic Rats Using Trypsin & DNase Digestion Technique

• Ryou JH
• Paik HJ
• Park MG

Abstract

Trypsin and DNase digestion technique has become a retinal digestion technique for studying diabetic retinopathy. We tried osmotic digestion method with DNase and compared the quality of preparation of retina and microvascular change with trypsin digestion in normal and diabetic rats. Streptozotocin-induced diabetic rats were sacrificed at 9, 18, 27weeks in 6 rats. Right retinas were digested with 3% trypsin while left wer digested with 0.1% DNase. For control, 18 normal rats were sacrificed at the same time. DNase was superior to trypsin for retinal preparation on the stainability, the degree of separating nonvascular fissue from vascular net, the degree of preservation of vascular net in normal & diabetic rats. In diabetic rats, the number of pericyte decreased significantly with age, which not in normal rats(p=0.0023) We suggest DNase digestion technique as an new alternative for trypsin digestion technique in the study of microvascular change of diabetic retinopathy.

Keyword

Diabetes mellitus; DNase; Endothelial cell; pericyte; Trypsin

MeSH Terms

Animals
Deoxyribonucleases*
Diabetes Mellitus
Diabetic Retinopathy
Digestion*
Endothelial Cells
Microvessels*
Pericytes
Rats*
Retina
Retinaldehyde*
Trypsin*
Deoxyribonucleases
Retinaldehyde
Trypsin