J Adv Prosthodont.  2015 Aug;7(4):278-287. 10.4047/jap.2015.7.4.278.

Color stability, water sorption and cytotoxicity of thermoplastic acrylic resin for non metal clasp denture

• Jang DE
• Lee JY
• Jang HS
• Lee JJ
• Son MK

• KMID: 2118263
• DOI: http://doi.org/10.4047/jap.2015.7.4.278

Abstract

PURPOSE
The aim of this study was to compare the color stability, water sorption and cytotoxicity of thermoplastic acrylic resin for the non-metal clasp dentures to those of thermoplastic polyamide and conventional heat-polymerized denture base resins.
MATERIALS AND METHODS
Three types of denture base resin, which are conventional heat-polymerized acrylic resin (Paladent 20), thermoplastic polyamide resin (Bio Tone), thermoplastic acrylic resin (Acrytone) were used as materials for this study. One hundred five specimens were fabricated. For the color stability test, specimens were immersed in the coffee and green tee for 1 and 8 weeks. Color change was measured by spectrometer. Water sorption was tested after 1 and 8 weeks immersion in the water. For the test of cytotoxicity, cell viability assay was measured and cell attachment was analyzed by FE-SEM.
RESULTS
All types of denture base resin showed color changes after 1 and 8 weeks immersion. However, there was no significant difference between denture base resins. All specimens showed significant color changes in the coffee than green tee. In water sorption test, thermoplastic acrylic resin showed lower values than conventional heat-polymerized acrylic resin and thermoplastic polyamide resin. Three types of denture base showed low cytotoxicity in cell viability assay. Thermoplastic acrylic resin showed the similar cell attachment but more stable attachment than conventional heat-polymerized acrylic resin.
CONCLUSION
Thermoplastic acrylic resin for the non-metal clasp denture showed acceptable color stability, water sorption and cytotoxicity. To verify the long stability in the mouth, additional in vitro studies are needed.

Keyword

Denture base; Color stability; Water sorption; Cytotoxicity

MeSH Terms

Cell Survival
Coffee
Denture Bases
Dentures*
Immersion
Mouth
Nylons
Water*
Coffee
Nylons
Water

Figure

• 

  Fig. 1 Result of cell viability assay after 1 and 6 days of the hGF cultured with denture base resins.

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  Fig. 2 FE-SEM images of the specimen's surface before cell attachments (×1,000). (A) Paladent 20, (B) Bio Tone, (C) Acrytone. The specimen of Bio Tone showed the smoothest surface. The specimen of Paladent 20 showed rough surface patterns and small pores.

• 

  Fig. 3 FE-SEM images of hGF cultured on the specimens after 1 day (×1,000). (A) Paladent 20, (B) Bio Tone, (C) Acrytone. The surface of Bio Tone showed most abundant hGF cell attachments. The surface of Acrytone showed moderate cell attachment appearance, and Paladent 20 showed a relatively weak cell attachments.

• 

  Fig. 4 FE-SEM images of hGF cultured on the specimens after 6 days (×1,000). (A) Paladent 20, (B) Bio Tone, (C) Acrytone. The surface of Bio Tone showed the most abundant hGF cell attachments. The hGF showed a multi-layer morphology on the Bio Tone surface.

• 

  Fig. 5 FE-SEM images of hGF cultured on the specimens after 10 days (×1,000). (A) Paladent 20, (B) Bio Tone, (C) Acrytone. The surface of Bio Tone showed the most abundant hGF cell attachments. The hGF showed the similar attachment on the Acrytone and Paladent 20. However, the hGF cultured on the Acrytone showed more stable attachment than on the Paladent 20.

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